Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles.
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| Title: | Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles. |
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| Authors: | Deng, Hao-Hua1,2, Hong, Guo-Lin3, Lin, Feng-Lin1,2, Liu, Ai-Lin1,2, Xia, Xing-Hua4, Chen, Wei1,2 chenandhu@163.com |
| Source: | Analytica Chimica Acta. Apr2016, Vol. 915, p74-80. 7p. |
| Subjects: | Colorimetric analysis, Urease, Chemical inhibitors, Gold nanoparticles, Horseradish peroxidase, Benzidine, Complex compounds |
| Abstract: | Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40–6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 μM and 1.8 U/L, respectively. The half-maximal inhibition value IC 50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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