A simple homemade spectrophotometer.

Saved in:
Bibliographic Details
Title: A simple homemade spectrophotometer.
Authors: Taha, Sara1, Rafat, Gelan1, Aboshosha, Fatma1, Mansour, Fotouh1 fotouhrashed@pharm.tanta.edu.eg
Source: Journal of Analytical Chemistry. Feb2017, Vol. 72 Issue 2, p239-242. 4p.
Subjects: Spectrophotometers, Absorbance scale (Spectroscopy), Salicylates, Alendronate, Phenolphthalein
Abstract: A simple portable spectrophotometer was constructed and used to perform analytical experiments. The developed model uses available tools and materials such as light emitting diode lamps, compact discs, plastic and cardboard boxes and a cell phone camera to build a design that illustrates the major components of spectrophotometers. The spectra of serial concentrations of KMnO (50-600 μM) were recorded and the values of absorbance were extracted at λ to build the calibration curve. A linear relationship between the concentration and absorbance was obtained with the coefficient of determination 0.994. The model was also utilized to study the spectra of KMnO, phenolphthalein and bromothymol blue in comparison with a Shimadzu UV-1800 spectrophotometer as a reference instrument. In spite of the differences in the observed spectra, the recorded λ were almost identical to those measured by the developed model. The model was successfully used to determine the concentration of sodium alendronate through ligand-exchange complexation with ferric salicylate. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Analytical Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:A simple portable spectrophotometer was constructed and used to perform analytical experiments. The developed model uses available tools and materials such as light emitting diode lamps, compact discs, plastic and cardboard boxes and a cell phone camera to build a design that illustrates the major components of spectrophotometers. The spectra of serial concentrations of KMnO (50-600 μM) were recorded and the values of absorbance were extracted at λ to build the calibration curve. A linear relationship between the concentration and absorbance was obtained with the coefficient of determination 0.994. The model was also utilized to study the spectra of KMnO, phenolphthalein and bromothymol blue in comparison with a Shimadzu UV-1800 spectrophotometer as a reference instrument. In spite of the differences in the observed spectra, the recorded λ were almost identical to those measured by the developed model. The model was successfully used to determine the concentration of sodium alendronate through ligand-exchange complexation with ferric salicylate. [ABSTRACT FROM AUTHOR]
ISSN:10619348
DOI:10.1134/S1061934817020113