Flow cytometric immunoassay for aflatoxin B1 using magnetic microspheres encoded with upconverting fluorescent nanocrystals.

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Bibliographic Details
Title:	Flow cytometric immunoassay for aflatoxin B1 using magnetic microspheres encoded with upconverting fluorescent nanocrystals.
Authors:	Zhang, Ying¹, Liao, Zhenyu² liaozy08@163.com, Liu, Yajuan¹, Wan, Yajuan³, Chang, Jin¹, Wang, Hanjie¹ wanghj@tju.edu.cn
Source:	Microchimica Acta. May2017, Vol. 184 Issue 5, p1471-1479. 9p.
Subjects:	Flow cytometry, Aflatoxins, Microspheres, Photon upconversion, Optical properties of nanocrystals
Abstract:	The authors describe a flow cytometric immunoassay for aflatoxin B1 (AFB1). It has three distinct features: (a) Magnetic microspheres encoded with upconverting nanocrystals (UCNMMs) are used as fluorescent labels. These have the advantage of non-overlapping spectra and lacking crosstalk between the encoding signal and reporter signal via the low-energy near-infrared (NIR) light excitation; (b) phycoerythrin-labeled secondary antibodies are used to amplify the reporter signal; (c) The use of magnetic nanoparticles facilitates the rapid separation and specific purification of the analyte (AFB1). This assay has a detection limit of 9 pg·mL and a broad working range for AFB1, requires a 50 μL sample only, and can be completed within 2 h with good accuracy and high reproducibility. It is perceived that such multifluorescent UCNMMs, whose color depends on the kind of dopants (Yb, Er, Tm, Mn) in the NaYF host lattice, represent a promising tool for the analysis of mycotoxins and other analytes. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	The authors describe a flow cytometric immunoassay for aflatoxin B1 (AFB1). It has three distinct features: (a) Magnetic microspheres encoded with upconverting nanocrystals (UCNMMs) are used as fluorescent labels. These have the advantage of non-overlapping spectra and lacking crosstalk between the encoding signal and reporter signal via the low-energy near-infrared (NIR) light excitation; (b) phycoerythrin-labeled secondary antibodies are used to amplify the reporter signal; (c) The use of magnetic nanoparticles facilitates the rapid separation and specific purification of the analyte (AFB1). This assay has a detection limit of 9 pg·mL and a broad working range for AFB1, requires a 50 μL sample only, and can be completed within 2 h with good accuracy and high reproducibility. It is perceived that such multifluorescent UCNMMs, whose color depends on the kind of dopants (Yb, Er, Tm, Mn) in the NaYF host lattice, represent a promising tool for the analysis of mycotoxins and other analytes. [ABSTRACT FROM AUTHOR]
ISSN:	00263672
DOI:	10.1007/s00604-017-2116-4