Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions.
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| Title: | Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions. |
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| Authors: | Cheng, Sy-Chyi1, Bhat, Suhail1, Shiea, Jentaie jetea@mail.nsysu.edu.tw |
| Source: | Journal of the American Society for Mass Spectrometry. Jul2017, Vol. 28 Issue 7, p1473-1481. 9p. |
| Subjects: | Chemical ionization mass spectrometry, Ion-molecule collisions, Atmospheric pressure, Nitrates, Amino acids |
| Abstract: | Flame atmospheric pressure chemical ionization (FAPCI) combined with negative electrospray ionization (ESI) mass spectrometry was developed to detect the ion/molecule reactions (IMRs) products between nitric acid (HNO) and negatively charged amino acid, angiotensin I (AI) and angiotensin II (AII), and insulin ions. Nitrate and HNO-nitrate ions were detected in the oxyacetylene flame, suggesting that a large quantity of nitric acid (HNO) was produced in the flame. The HNO and negatively charged analyte ions produced by a negative ESI source were delivered into each arm of a Y-shaped stainless steel tube where they merged and reacted. The products were subsequently characterized with an ion trap mass analyzer attached to the exit of the Y-tube. HNO showed the strongest affinity to histidine and formed (M-H+HNO) complex ions, whereas some amino acids did not react with HNO at all. Reactions between HNO and histidine residues in AI and AII resulted in the formation of dominant [M-H+(HNO)] and [M-H+(HNO)] ions. Results from analyses of AAs and insulin indicated that HNO could not only react with basic amino acid residues, but also with disulfide bonds to form [M-3H+(HNO)] complex ions. This approach is useful for obtaining information about the number of basic amino acid residues and disulfide bonds in peptides and proteins. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 123773113 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Cheng%2C+Sy-Chyi%22">Cheng, Sy-Chyi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bhat%2C+Suhail%22">Bhat, Suhail</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shiea%2C+Jentaie%22">Shiea, Jentaie</searchLink><i> jetea@mail.nsysu.edu.tw</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+the+American+Society+for+Mass+Spectrometry%22">Journal of the American Society for Mass Spectrometry</searchLink>. Jul2017, Vol. 28 Issue 7, p1473-1481. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Chemical+ionization+mass+spectrometry%22">Chemical ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Ion-molecule+collisions%22">Ion-molecule collisions</searchLink><br /><searchLink fieldCode="DE" term="%22Atmospheric+pressure%22">Atmospheric pressure</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrates%22">Nitrates</searchLink><br /><searchLink fieldCode="DE" term="%22Amino+acids%22">Amino acids</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Flame atmospheric pressure chemical ionization (FAPCI) combined with negative electrospray ionization (ESI) mass spectrometry was developed to detect the ion/molecule reactions (IMRs) products between nitric acid (HNO) and negatively charged amino acid, angiotensin I (AI) and angiotensin II (AII), and insulin ions. Nitrate and HNO-nitrate ions were detected in the oxyacetylene flame, suggesting that a large quantity of nitric acid (HNO) was produced in the flame. The HNO and negatively charged analyte ions produced by a negative ESI source were delivered into each arm of a Y-shaped stainless steel tube where they merged and reacted. The products were subsequently characterized with an ion trap mass analyzer attached to the exit of the Y-tube. HNO showed the strongest affinity to histidine and formed (M-H+HNO) complex ions, whereas some amino acids did not react with HNO at all. Reactions between HNO and histidine residues in AI and AII resulted in the formation of dominant [M-H+(HNO)] and [M-H+(HNO)] ions. Results from analyses of AAs and insulin indicated that HNO could not only react with basic amino acid residues, but also with disulfide bonds to form [M-3H+(HNO)] complex ions. This approach is useful for obtaining information about the number of basic amino acid residues and disulfide bonds in peptides and proteins. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s13361-017-1688-x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1473 Subjects: – SubjectFull: Chemical ionization mass spectrometry Type: general – SubjectFull: Ion-molecule collisions Type: general – SubjectFull: Atmospheric pressure Type: general – SubjectFull: Nitrates Type: general – SubjectFull: Amino acids Type: general Titles: – TitleFull: Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Cheng, Sy-Chyi – PersonEntity: Name: NameFull: Bhat, Suhail – PersonEntity: Name: NameFull: Shiea, Jentaie IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 10440305 Numbering: – Type: volume Value: 28 – Type: issue Value: 7 Titles: – TitleFull: Journal of the American Society for Mass Spectrometry Type: main |
| ResultId | 1 |
