Bibliographic Details
| Title: |
Generation of Arabidopsis thaliana plants with complex N-glycans lacking β1,2-linked xylose and core α1,3-linked fucose |
| Authors: |
Strasser, R.1 richard.strasser@boku.ac.at, Altmann, F.2, Mach, L.1, Glössl, J.1, Steinkellner, H.1 |
| Source: |
FEBS Letters. Mar2004, Vol. 561 Issue 1-3, p132. 5p. |
| Subjects: |
Fucosyltransferases, Glycosyltransferases, Plant enzymes, Arabidopsis thaliana |
| Abstract: |
The plant glycosyltransferases, β1,2-xylosyltransferase (XylT) and core α1,3-fucosyltransferase (FucT), are responsible for the transfer of β1,2-linked xylose and core α1,3-linked fucose residues to glycoprotein N-glycans. These glycan epitopes are not present in humans and thus may cause immunological responses, which represent a limitation for the therapeutic use of recombinant mammalian glycoproteins produced in transgenic plants. Here we report the genetic modification of the N-glycosylation pathway in Arabidopsis thaliana plants. Knockout plants were generated with complete deficiency of XylT and FucT. These plants lack antigenic protein-bound N-glycans and instead synthesise predominantly structures with two terminal βN-acetylglucosamine residues (GlcNAc2Man3GlcNAc2). [Copyright &y& Elsevier] |
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| Database: |
Engineering Source |
