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Effective production of n-butanol in Escherichia coli utilizing the glucose–glycerol mixture.

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Title: Effective production of n-butanol in Escherichia coli utilizing the glucose–glycerol mixture.
Authors: Saini, Mukesh1, Lin, Li-Jen2, Chiang, Chung-Jen3 cjchiang@mail.cmu.edu.tw, Chao, Yun-Peng1,4,5 ypchao@fcu.edu.tw
Source: Journal of the Taiwan Institute of Chemical Engineers. Dec2017, Vol. 81, p134-139. 6p.
Subjects: Butanol, Escherichia coli biotechnology, Mixtures, Glucose, Glycerin, Strain theory (Chemistry)
Abstract: Glucose from plant cellulose and glycerol from the waste stream of the biodiesel production process are two most abundant and renewable carbon sources. n -Butanol is an alternative fuel of potential and its production by microbes which utilize the two carbons appears to be industrially incentive on the ground of sustainability and economy. However, the hierarchical regulation of glucose dictates Escherichia coli to prefer utilization of glucose over others. This physiological behavior of the cell prolongs the fermentation process and eventually reduces productivity. In this study, a single strain capable of co-utilizing glucose and glycerol was developed by metabolic engineering. Meanwhile, a dual-culture system was also designed by construction of a glucose-selecting and a glycerol-selecting strain. As a consequence, both approaches result in the strains for effective co-utilization of glucose and glycerol. In particular, the single strain enabled production of 6.2 g/L n -butanol, which leads to the conversion yield and productivity accounting for 76.5% of the theoretical and 0.17 g/L/h, respectively. It indicates a promise of the developed technology platform for microbial production of n -butanol from the glucose–glycerol mixture. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Glucose from plant cellulose and glycerol from the waste stream of the biodiesel production process are two most abundant and renewable carbon sources. n -Butanol is an alternative fuel of potential and its production by microbes which utilize the two carbons appears to be industrially incentive on the ground of sustainability and economy. However, the hierarchical regulation of glucose dictates Escherichia coli to prefer utilization of glucose over others. This physiological behavior of the cell prolongs the fermentation process and eventually reduces productivity. In this study, a single strain capable of co-utilizing glucose and glycerol was developed by metabolic engineering. Meanwhile, a dual-culture system was also designed by construction of a glucose-selecting and a glycerol-selecting strain. As a consequence, both approaches result in the strains for effective co-utilization of glucose and glycerol. In particular, the single strain enabled production of 6.2 g/L n -butanol, which leads to the conversion yield and productivity accounting for 76.5% of the theoretical and 0.17 g/L/h, respectively. It indicates a promise of the developed technology platform for microbial production of n -butanol from the glucose–glycerol mixture. [ABSTRACT FROM AUTHOR]
ISSN:18761070
DOI:10.1016/j.jtice.2017.09.039