Effective production of n-butanol in Escherichia coli utilizing the glucose–glycerol mixture.
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| Title: | Effective production of n-butanol in Escherichia coli utilizing the glucose–glycerol mixture. |
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| Authors: | Saini, Mukesh1, Lin, Li-Jen2, Chiang, Chung-Jen3 cjchiang@mail.cmu.edu.tw, Chao, Yun-Peng1,4,5 ypchao@fcu.edu.tw |
| Source: | Journal of the Taiwan Institute of Chemical Engineers. Dec2017, Vol. 81, p134-139. 6p. |
| Subjects: | Butanol, Escherichia coli biotechnology, Mixtures, Glucose, Glycerin, Strain theory (Chemistry) |
| Abstract: | Glucose from plant cellulose and glycerol from the waste stream of the biodiesel production process are two most abundant and renewable carbon sources. n -Butanol is an alternative fuel of potential and its production by microbes which utilize the two carbons appears to be industrially incentive on the ground of sustainability and economy. However, the hierarchical regulation of glucose dictates Escherichia coli to prefer utilization of glucose over others. This physiological behavior of the cell prolongs the fermentation process and eventually reduces productivity. In this study, a single strain capable of co-utilizing glucose and glycerol was developed by metabolic engineering. Meanwhile, a dual-culture system was also designed by construction of a glucose-selecting and a glycerol-selecting strain. As a consequence, both approaches result in the strains for effective co-utilization of glucose and glycerol. In particular, the single strain enabled production of 6.2 g/L n -butanol, which leads to the conversion yield and productivity accounting for 76.5% of the theoretical and 0.17 g/L/h, respectively. It indicates a promise of the developed technology platform for microbial production of n -butanol from the glucose–glycerol mixture. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of the Taiwan Institute of Chemical Engineers is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 126350358 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effective production of n-butanol in Escherichia coli utilizing the glucose–glycerol mixture. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Saini%2C+Mukesh%22">Saini, Mukesh</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lin%2C+Li-Jen%22">Lin, Li-Jen</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Chiang%2C+Chung-Jen%22">Chiang, Chung-Jen</searchLink><relatesTo>3</relatesTo><i> cjchiang@mail.cmu.edu.tw</i><br /><searchLink fieldCode="AR" term="%22Chao%2C+Yun-Peng%22">Chao, Yun-Peng</searchLink><relatesTo>1,4,5</relatesTo><i> ypchao@fcu.edu.tw</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+the+Taiwan+Institute+of+Chemical+Engineers%22">Journal of the Taiwan Institute of Chemical Engineers</searchLink>. Dec2017, Vol. 81, p134-139. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Butanol%22">Butanol</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli+biotechnology%22">Escherichia coli biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Mixtures%22">Mixtures</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose%22">Glucose</searchLink><br /><searchLink fieldCode="DE" term="%22Glycerin%22">Glycerin</searchLink><br /><searchLink fieldCode="DE" term="%22Strain+theory+%28Chemistry%29%22">Strain theory (Chemistry)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Glucose from plant cellulose and glycerol from the waste stream of the biodiesel production process are two most abundant and renewable carbon sources. n -Butanol is an alternative fuel of potential and its production by microbes which utilize the two carbons appears to be industrially incentive on the ground of sustainability and economy. However, the hierarchical regulation of glucose dictates Escherichia coli to prefer utilization of glucose over others. This physiological behavior of the cell prolongs the fermentation process and eventually reduces productivity. In this study, a single strain capable of co-utilizing glucose and glycerol was developed by metabolic engineering. Meanwhile, a dual-culture system was also designed by construction of a glucose-selecting and a glycerol-selecting strain. As a consequence, both approaches result in the strains for effective co-utilization of glucose and glycerol. In particular, the single strain enabled production of 6.2 g/L n -butanol, which leads to the conversion yield and productivity accounting for 76.5% of the theoretical and 0.17 g/L/h, respectively. It indicates a promise of the developed technology platform for microbial production of n -butanol from the glucose–glycerol mixture. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of the Taiwan Institute of Chemical Engineers is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jtice.2017.09.039 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 134 Subjects: – SubjectFull: Butanol Type: general – SubjectFull: Escherichia coli biotechnology Type: general – SubjectFull: Mixtures Type: general – SubjectFull: Glucose Type: general – SubjectFull: Glycerin Type: general – SubjectFull: Strain theory (Chemistry) Type: general Titles: – TitleFull: Effective production of n-butanol in Escherichia coli utilizing the glucose–glycerol mixture. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Saini, Mukesh – PersonEntity: Name: NameFull: Lin, Li-Jen – PersonEntity: Name: NameFull: Chiang, Chung-Jen – PersonEntity: Name: NameFull: Chao, Yun-Peng IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 18761070 Numbering: – Type: volume Value: 81 Titles: – TitleFull: Journal of the Taiwan Institute of Chemical Engineers Type: main |
| ResultId | 1 |
