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Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1.

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Title: Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1.
Authors: You, Le-Xing1,2, Liu, Li-Dan1,3, Xiao, Yong1,4 yxiao@iue.ac.cn, Dai, You-Fen1,3, Chen, Bi-Lian3, Jiang, Yan-Xia2, Zhao, Feng1
Source: Bioelectrochemistry. Feb2018, Vol. 119, p196-202. 7p.
Subjects: Flavins, Charge exchange, Bacillus megaterium, Ribosomal RNA, Electrochemistry
Abstract: The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E - t and amperometric I - t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E - t and amperometric I - t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed. [ABSTRACT FROM AUTHOR]
ISSN:15675394
DOI:10.1016/j.bioelechem.2017.10.005