Bibliographic Details
| Title: |
Stable Isotope-Resolved Analysis with Quantitative Dissolution Dynamic Nuclear Polarization. |
| Authors: |
Lerche, Mathilde H.1, Yigit, Demet1, Frahm, Anne B.1, Ardenkjær-Larsen, Jan Henrik1, Malinowski, Ronja M.1, Jensen, Pernille R.1 peroje@elektro.dtu.dk |
| Source: |
Analytical Chemistry. 1/2/2018, Vol. 90 Issue 1, p674-678. 5p. |
| Subjects: |
Polarization (Nuclear physics), Metabolites, Nuclear magnetic resonance, Cancer cells, Stable isotopes |
| Abstract: |
Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such metabolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ signal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhancement, to obtain reproducible and quantitative dDNP (qdDNP) NMR-based stable isotope-resolved analysis. We further illustrate how qdDNP was applied to gain metabolic insights into the phenotype of aggressive cancer cells. [ABSTRACT FROM AUTHOR] |
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| Database: |
Engineering Source |
