View in EDS

Stable Isotope-Resolved Analysis with Quantitative Dissolution Dynamic Nuclear Polarization.

Saved in:
Bibliographic Details
Title: Stable Isotope-Resolved Analysis with Quantitative Dissolution Dynamic Nuclear Polarization.
Authors: Lerche, Mathilde H.1, Yigit, Demet1, Frahm, Anne B.1, Ardenkjær-Larsen, Jan Henrik1, Malinowski, Ronja M.1, Jensen, Pernille R.1 peroje@elektro.dtu.dk
Source: Analytical Chemistry. 1/2/2018, Vol. 90 Issue 1, p674-678. 5p.
Subjects: Polarization (Nuclear physics), Metabolites, Nuclear magnetic resonance, Cancer cells, Stable isotopes
Abstract: Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such metabolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ signal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhancement, to obtain reproducible and quantitative dDNP (qdDNP) NMR-based stable isotope-resolved analysis. We further illustrate how qdDNP was applied to gain metabolic insights into the phenotype of aggressive cancer cells. [ABSTRACT FROM AUTHOR]
Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 127144155
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Stable Isotope-Resolved Analysis with Quantitative Dissolution Dynamic Nuclear Polarization.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Lerche%2C+Mathilde+H%2E%22">Lerche, Mathilde H.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yigit%2C+Demet%22">Yigit, Demet</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Frahm%2C+Anne+B%2E%22">Frahm, Anne B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ardenkjær-Larsen%2C+Jan+Henrik%22">Ardenkjær-Larsen, Jan Henrik</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Malinowski%2C+Ronja+M%2E%22">Malinowski, Ronja M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jensen%2C+Pernille+R%2E%22">Jensen, Pernille R.</searchLink><relatesTo>1</relatesTo><i> peroje@elektro.dtu.dk</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Analytical+Chemistry%22">Analytical Chemistry</searchLink>. 1/2/2018, Vol. 90 Issue 1, p674-678. 5p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Polarization+%28Nuclear+physics%29%22">Polarization (Nuclear physics)</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolites%22">Metabolites</searchLink><br /><searchLink fieldCode="DE" term="%22Nuclear+magnetic+resonance%22">Nuclear magnetic resonance</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+cells%22">Cancer cells</searchLink><br /><searchLink fieldCode="DE" term="%22Stable+isotopes%22">Stable isotopes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such metabolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ signal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhancement, to obtain reproducible and quantitative dDNP (qdDNP) NMR-based stable isotope-resolved analysis. We further illustrate how qdDNP was applied to gain metabolic insights into the phenotype of aggressive cancer cells. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=127144155
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1021/acs.analchem.7b02779
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 5
        StartPage: 674
    Subjects:
      – SubjectFull: Polarization (Nuclear physics)
        Type: general
      – SubjectFull: Metabolites
        Type: general
      – SubjectFull: Nuclear magnetic resonance
        Type: general
      – SubjectFull: Cancer cells
        Type: general
      – SubjectFull: Stable isotopes
        Type: general
    Titles:
      – TitleFull: Stable Isotope-Resolved Analysis with Quantitative Dissolution Dynamic Nuclear Polarization.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Lerche, Mathilde H.
      – PersonEntity:
          Name:
            NameFull: Yigit, Demet
      – PersonEntity:
          Name:
            NameFull: Frahm, Anne B.
      – PersonEntity:
          Name:
            NameFull: Ardenkjær-Larsen, Jan Henrik
      – PersonEntity:
          Name:
            NameFull: Malinowski, Ronja M.
      – PersonEntity:
          Name:
            NameFull: Jensen, Pernille R.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 02
              M: 01
              Text: 1/2/2018
              Type: published
              Y: 2018
          Identifiers:
            – Type: issn-print
              Value: 00032700
          Numbering:
            – Type: volume
              Value: 90
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Analytical Chemistry
              Type: main
ResultId 1