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A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration.

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Title: A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration.
Authors: Podder, Arup1, Won, Miae2, Kim, Soobin2, Verwilst, Peter2, Maiti, Mrinmoy1, Yang, Zhigang3, Qu, Junle3 jlqu@szu.edu.cn, Bhuniya, Sankarprasad1,4 b_sankarprasad@cb.amrita.edu, Kim, Jong Seung2 jongskim@korea.ac.kr
Source: Sensors & Actuators B: Chemical. Sep2018, Vol. 268, p195-204. 10p.
Subjects: Oxonium ions, Pyridyl compounds, Hydrogen-ion concentration, Reactive oxygen species, Mitochondria
Abstract: Mapping the intracellular location and concentration of hydronium ions (H 3 O + ) and their dynamics could be a useful diagnostic tool in modern clinical science. Current needs motivated us to develop a molecular pH probe 1 , operating as a logic gate, and its analogue 2 . The pyridyl moiety in 1 played a significant role in proton capture and release, in acidic to alkaline pH environments. In contrast, 2 failed to show a similar spectroscopic behavior. 1 shows emission maximum at 450 nm that is independent on the pH, with excitation at 353 nm or 410 nm in acid and alkaline pH, respectively. 1 was employed to provide input-dependent (excitation wavelength) fluorescence images in a cellular milieu to detect pH changes in cellular organelles such as lysosomes and mitochondria. Furthermore, 1 provided information on the variation of the pH in the presence of cellular ROS. 1 was also found to enable the real-time monitoring of cell acidification due to nutrient starvation, which is closely associated with mitochondrial malfunction, fusion and mitophagy processes. We envision that in due course 1 can open up new research avenues in the diagnostic sector for validating the pH in the cellular milieu. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Mapping the intracellular location and concentration of hydronium ions (H 3 O + ) and their dynamics could be a useful diagnostic tool in modern clinical science. Current needs motivated us to develop a molecular pH probe 1 , operating as a logic gate, and its analogue 2 . The pyridyl moiety in 1 played a significant role in proton capture and release, in acidic to alkaline pH environments. In contrast, 2 failed to show a similar spectroscopic behavior. 1 shows emission maximum at 450 nm that is independent on the pH, with excitation at 353 nm or 410 nm in acid and alkaline pH, respectively. 1 was employed to provide input-dependent (excitation wavelength) fluorescence images in a cellular milieu to detect pH changes in cellular organelles such as lysosomes and mitochondria. Furthermore, 1 provided information on the variation of the pH in the presence of cellular ROS. 1 was also found to enable the real-time monitoring of cell acidification due to nutrient starvation, which is closely associated with mitochondrial malfunction, fusion and mitophagy processes. We envision that in due course 1 can open up new research avenues in the diagnostic sector for validating the pH in the cellular milieu. [ABSTRACT FROM AUTHOR]
ISSN:09254005
DOI:10.1016/j.snb.2018.04.092