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A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration.

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Title: A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration.
Authors: Podder, Arup1, Won, Miae2, Kim, Soobin2, Verwilst, Peter2, Maiti, Mrinmoy1, Yang, Zhigang3, Qu, Junle3 jlqu@szu.edu.cn, Bhuniya, Sankarprasad1,4 b_sankarprasad@cb.amrita.edu, Kim, Jong Seung2 jongskim@korea.ac.kr
Source: Sensors & Actuators B: Chemical. Sep2018, Vol. 268, p195-204. 10p.
Subjects: Oxonium ions, Pyridyl compounds, Hydrogen-ion concentration, Reactive oxygen species, Mitochondria
Abstract: Mapping the intracellular location and concentration of hydronium ions (H 3 O + ) and their dynamics could be a useful diagnostic tool in modern clinical science. Current needs motivated us to develop a molecular pH probe 1 , operating as a logic gate, and its analogue 2 . The pyridyl moiety in 1 played a significant role in proton capture and release, in acidic to alkaline pH environments. In contrast, 2 failed to show a similar spectroscopic behavior. 1 shows emission maximum at 450 nm that is independent on the pH, with excitation at 353 nm or 410 nm in acid and alkaline pH, respectively. 1 was employed to provide input-dependent (excitation wavelength) fluorescence images in a cellular milieu to detect pH changes in cellular organelles such as lysosomes and mitochondria. Furthermore, 1 provided information on the variation of the pH in the presence of cellular ROS. 1 was also found to enable the real-time monitoring of cell acidification due to nutrient starvation, which is closely associated with mitochondrial malfunction, fusion and mitophagy processes. We envision that in due course 1 can open up new research avenues in the diagnostic sector for validating the pH in the cellular milieu. [ABSTRACT FROM AUTHOR]
Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 129507983
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  Data: A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration.
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  Data: <searchLink fieldCode="AR" term="%22Podder%2C+Arup%22">Podder, Arup</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Won%2C+Miae%22">Won, Miae</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kim%2C+Soobin%22">Kim, Soobin</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Verwilst%2C+Peter%22">Verwilst, Peter</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Maiti%2C+Mrinmoy%22">Maiti, Mrinmoy</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang%2C+Zhigang%22">Yang, Zhigang</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Qu%2C+Junle%22">Qu, Junle</searchLink><relatesTo>3</relatesTo><i> jlqu@szu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Bhuniya%2C+Sankarprasad%22">Bhuniya, Sankarprasad</searchLink><relatesTo>1,4</relatesTo><i> b_sankarprasad@cb.amrita.edu</i><br /><searchLink fieldCode="AR" term="%22Kim%2C+Jong+Seung%22">Kim, Jong Seung</searchLink><relatesTo>2</relatesTo><i> jongskim@korea.ac.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Sensors+%26+Actuators+B%3A+Chemical%22">Sensors & Actuators B: Chemical</searchLink>. Sep2018, Vol. 268, p195-204. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Oxonium+ions%22">Oxonium ions</searchLink><br /><searchLink fieldCode="DE" term="%22Pyridyl+compounds%22">Pyridyl compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrogen-ion+concentration%22">Hydrogen-ion concentration</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondria%22">Mitochondria</searchLink>
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  Data: Mapping the intracellular location and concentration of hydronium ions (H 3 O + ) and their dynamics could be a useful diagnostic tool in modern clinical science. Current needs motivated us to develop a molecular pH probe 1 , operating as a logic gate, and its analogue 2 . The pyridyl moiety in 1 played a significant role in proton capture and release, in acidic to alkaline pH environments. In contrast, 2 failed to show a similar spectroscopic behavior. 1 shows emission maximum at 450 nm that is independent on the pH, with excitation at 353 nm or 410 nm in acid and alkaline pH, respectively. 1 was employed to provide input-dependent (excitation wavelength) fluorescence images in a cellular milieu to detect pH changes in cellular organelles such as lysosomes and mitochondria. Furthermore, 1 provided information on the variation of the pH in the presence of cellular ROS. 1 was also found to enable the real-time monitoring of cell acidification due to nutrient starvation, which is closely associated with mitochondrial malfunction, fusion and mitophagy processes. We envision that in due course 1 can open up new research avenues in the diagnostic sector for validating the pH in the cellular milieu. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.snb.2018.04.092
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      – Code: eng
        Text: English
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        PageCount: 10
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      – SubjectFull: Oxonium ions
        Type: general
      – SubjectFull: Pyridyl compounds
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      – SubjectFull: Hydrogen-ion concentration
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      – SubjectFull: Reactive oxygen species
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      – SubjectFull: Mitochondria
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      – TitleFull: A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration.
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            NameFull: Podder, Arup
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            NameFull: Won, Miae
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            – D: 01
              M: 09
              Text: Sep2018
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              Y: 2018
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              Value: 268
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