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Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis.

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Bibliographic Details
Title: Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis.
Authors: Zhou, Li-Ping1, Chen, Yao1, Hu, Hao1, Yu, Bing1, Wang, Guo-Wei2, Cong, Hai-Lin1 yubingcong198@yahoo.com
Source: Ferroelectrics. 2018, Vol. 529 Issue 1, p24-32. 9p.
Subjects: Capillary electrophoresis, Biomacromolecules, Carboxymethyl compounds, Chitosan, Surface coatings, Photochemistry, Covalent bonds
Abstract: Capillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Capillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE. [ABSTRACT FROM AUTHOR]
ISSN:00150193
DOI:10.1080/00150193.2018.1448184