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Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis.

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Title: Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis.
Authors: Zhou, Li-Ping1, Chen, Yao1, Hu, Hao1, Yu, Bing1, Wang, Guo-Wei2, Cong, Hai-Lin1 yubingcong198@yahoo.com
Source: Ferroelectrics. 2018, Vol. 529 Issue 1, p24-32. 9p.
Subjects: Capillary electrophoresis, Biomacromolecules, Carboxymethyl compounds, Chitosan, Surface coatings, Photochemistry, Covalent bonds
Abstract: Capillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE. [ABSTRACT FROM AUTHOR]
Copyright of Ferroelectrics is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 130320883
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Label: Title
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  Data: Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis.
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  Data: <searchLink fieldCode="AR" term="%22Zhou%2C+Li-Ping%22">Zhou, Li-Ping</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Yao%22">Chen, Yao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hu%2C+Hao%22">Hu, Hao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yu%2C+Bing%22">Yu, Bing</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Guo-Wei%22">Wang, Guo-Wei</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Cong%2C+Hai-Lin%22">Cong, Hai-Lin</searchLink><relatesTo>1</relatesTo><i> yubingcong198@yahoo.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Ferroelectrics%22">Ferroelectrics</searchLink>. 2018, Vol. 529 Issue 1, p24-32. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Capillary+electrophoresis%22">Capillary electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Biomacromolecules%22">Biomacromolecules</searchLink><br /><searchLink fieldCode="DE" term="%22Carboxymethyl+compounds%22">Carboxymethyl compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Chitosan%22">Chitosan</searchLink><br /><searchLink fieldCode="DE" term="%22Surface+coatings%22">Surface coatings</searchLink><br /><searchLink fieldCode="DE" term="%22Photochemistry%22">Photochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Covalent+bonds%22">Covalent bonds</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Capillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Ferroelectrics is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1080/00150193.2018.1448184
    Languages:
      – Code: eng
        Text: English
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        PageCount: 9
        StartPage: 24
    Subjects:
      – SubjectFull: Capillary electrophoresis
        Type: general
      – SubjectFull: Biomacromolecules
        Type: general
      – SubjectFull: Carboxymethyl compounds
        Type: general
      – SubjectFull: Chitosan
        Type: general
      – SubjectFull: Surface coatings
        Type: general
      – SubjectFull: Photochemistry
        Type: general
      – SubjectFull: Covalent bonds
        Type: general
    Titles:
      – TitleFull: Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis.
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          Name:
            NameFull: Zhou, Li-Ping
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            NameFull: Chen, Yao
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            NameFull: Hu, Hao
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            NameFull: Yu, Bing
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            NameFull: Wang, Guo-Wei
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            NameFull: Cong, Hai-Lin
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            – D: 15
              M: 04
              Text: 2018
              Type: published
              Y: 2018
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              Value: 529
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            – TitleFull: Ferroelectrics
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