Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus
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| Title: | Effects of hypoxia on gene and protein expression in the blue crab, Callinectes sapidus |
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| Authors: | Brouwer, Marius1 Marius.brouwer@usm.edu, Larkin, Patrick2,3, Brown-Peterson, Nancy1, King, Christina1, Manning, Steve1, Denslow, Nancy2 |
| Source: | Marine Environmental Research. Aug2004, Vol. 58 Issue 2-5, p787-792. 6p. |
| Subjects: | Crabs, Hypoxia (Water), Biomarkers |
| Abstract: | Increases in hypoxic conditions are one of the major factors responsible for declines in estuarine habitat quality, yet to date there are no indicators for recognizing populations of estuarine organisms that are suffering from chronic hypoxic stress. Here we test the hypothesis that alterations in gene and protein expression of antioxidant enzymes and other stress-specific proteins can be used as molecular indicators of hypoxic stress. Blue crabs, Callinectes sapidus, were exposed to 2–3 ppm DO for 5 days. Gene expression was measured using macroarrays constructed from cDNA of 10 partial gene transcripts cloned from blue crab hepatopancreas. Significant ( |
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| Database: | Engineering Source |
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