Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization.
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| Title: | Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization. |
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| Authors: | Zairov, Rustem R.1 rustem@iopc.ru, Solovieva, Anastasiya O.1, Shamsutdinova, Nataliya A.1, Podyachev, Sergey N.1, Shestopalov, Michael A.1, Pozmogova, Tatiana N.1, Miroshnichenko, Svetlana M.1, Mustafina, Asiya R.1, Karasik, Andrey A.1 |
| Source: | Materials Science & Engineering: C. Feb2019, Vol. 95, p166-173. 8p. |
| Subjects: | Polyelectrolytes, Luminescence, Nanoparticles, Terbium, Metal complexes, Energy transfer |
| Abstract: | Abstract The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels. Highlights • Polyelectrolyte-coated nanoparticles with green Tb-luminescence for cell imaging • Layer-by-layer technique for variation of the exterior polyelectrolyte coating • Recharging and de-aggregation of the nanoparticles in biological background • High viability of Hep-2 cells incubated by the nanoparticles • Impact of the exterior polyelectrolyte layer on the cell internalization [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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