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DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.

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Title: DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.
Authors: Xing, Ran-Ran1 (AUTHOR), Hu, Ran-Ran1,2 (AUTHOR), Han, Jian-Xun1,3 (AUTHOR), Deng, Ting-Ting1,2 (AUTHOR), Chen, Ying1 (AUTHOR) chenyingcaiq@163.com
Source: Food Chemistry. Mar2020, Vol. 309, pN.PAG-N.PAG. 1p.
Subjects: Genetic barcoding, Cytochrome oxidase, Market potential, Case studies, Processed foods, Poultry
Abstract: • • Both full- and mini-barcodes were evaluated in authenticating animal-derived food. • • Mini-barcoding was more efficient in authenticating processed food than full-barcoding. • • Mini-barcode has high potential, in combination with NGS, for detecting mislabeled food products. • • Approximately 23% of selected commercial samples were determined to be mislabeled. This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (~650 bp) and 16S rRNA (~220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI -failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:• • Both full- and mini-barcodes were evaluated in authenticating animal-derived food. • • Mini-barcoding was more efficient in authenticating processed food than full-barcoding. • • Mini-barcode has high potential, in combination with NGS, for detecting mislabeled food products. • • Approximately 23% of selected commercial samples were determined to be mislabeled. This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (~650 bp) and 16S rRNA (~220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI -failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted. [ABSTRACT FROM AUTHOR]
ISSN:03088146
DOI:10.1016/j.foodchem.2019.125653