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DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.

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Title: DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.
Authors: Xing, Ran-Ran1 (AUTHOR), Hu, Ran-Ran1,2 (AUTHOR), Han, Jian-Xun1,3 (AUTHOR), Deng, Ting-Ting1,2 (AUTHOR), Chen, Ying1 (AUTHOR) chenyingcaiq@163.com
Source: Food Chemistry. Mar2020, Vol. 309, pN.PAG-N.PAG. 1p.
Subjects: Genetic barcoding, Cytochrome oxidase, Market potential, Case studies, Processed foods, Poultry
Abstract: • • Both full- and mini-barcodes were evaluated in authenticating animal-derived food. • • Mini-barcoding was more efficient in authenticating processed food than full-barcoding. • • Mini-barcode has high potential, in combination with NGS, for detecting mislabeled food products. • • Approximately 23% of selected commercial samples were determined to be mislabeled. This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (~650 bp) and 16S rRNA (~220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI -failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted. [ABSTRACT FROM AUTHOR]
Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 140093624
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.
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  Data: <searchLink fieldCode="AR" term="%22Xing%2C+Ran-Ran%22">Xing, Ran-Ran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hu%2C+Ran-Ran%22">Hu, Ran-Ran</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Han%2C+Jian-Xun%22">Han, Jian-Xun</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Deng%2C+Ting-Ting%22">Deng, Ting-Ting</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Ying%22">Chen, Ying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> chenyingcaiq@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Food+Chemistry%22">Food Chemistry</searchLink>. Mar2020, Vol. 309, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Genetic+barcoding%22">Genetic barcoding</searchLink><br /><searchLink fieldCode="DE" term="%22Cytochrome+oxidase%22">Cytochrome oxidase</searchLink><br /><searchLink fieldCode="DE" term="%22Market+potential%22">Market potential</searchLink><br /><searchLink fieldCode="DE" term="%22Case+studies%22">Case studies</searchLink><br /><searchLink fieldCode="DE" term="%22Processed+foods%22">Processed foods</searchLink><br /><searchLink fieldCode="DE" term="%22Poultry%22">Poultry</searchLink>
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  Label: Abstract
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  Data: • • Both full- and mini-barcodes were evaluated in authenticating animal-derived food. • • Mini-barcoding was more efficient in authenticating processed food than full-barcoding. • • Mini-barcode has high potential, in combination with NGS, for detecting mislabeled food products. • • Approximately 23% of selected commercial samples were determined to be mislabeled. This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (~650 bp) and 16S rRNA (~220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI -failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.foodchem.2019.125653
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        Text: English
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      – SubjectFull: Cytochrome oxidase
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      – SubjectFull: Market potential
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      – SubjectFull: Processed foods
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      – TitleFull: DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.
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              Text: Mar2020
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