View in EDS

Boosting the biocatalytic precipitation with enzyme-loaded liposomes: Toward a general platform for amplified photoelectrochemical immunoassay.

Saved in:
Bibliographic Details
Title: Boosting the biocatalytic precipitation with enzyme-loaded liposomes: Toward a general platform for amplified photoelectrochemical immunoassay.
Authors: Yu, Li-Min1,2 (AUTHOR), Liu, Yi-Li1,2 (AUTHOR) liuyili2019@163.com, Zhu, Li-Bang2 (AUTHOR), Shen, Qi3 (AUTHOR), Han, De-Man2 (AUTHOR), Qu, Peng1 (AUTHOR) qupeng0212@163.com, Zhao, Wei-Wei1,2 (AUTHOR) zww@nju.edu.cn
Source: Analytica Chimica Acta. Jun2020, Vol. 1115, p1-6. 6p.
Subjects: Horseradish peroxidase, Immunoassay, Meteorological precipitation, Photoelectrochemical cells, Liposomes, Immune recognition, Peroxidase
Abstract: Liposome-assisted photoelectrochemical (PEC) bioanalysis represents one of the latest frontiers in the arena of PEC bioanalysis. This work reports a general enzyme-amplified liposomal PEC bioanalysis protocol via the use of enzyme-loaded liposomes to boost the biocatalytic precipitation (BCP) effect. In the representative system, the horseradish peroxidase (HRP)-loaded liposome (HRPLL) and the Au nanoclusters (NCs)/Au nanoparticles (NPs)/TiO 2 nanotubes (NTs) framework (AATF) were used as liposomal label and photoelectrode, respectively. In the detection, the sandwich immunocomplex reaction was accomplished in a 96-well plate to confine the HRPLL label, which was then lysed to release the HRP molecules to initiate the BCP process. Due to the amplified formation of HRP-induced BCP on the AATF scaffold, the photo-current response correlated closely with the immunorecognition process and the analyte could be detected very sensitively. This work features the first integration of enzyme-loaded liposomes and the BCP for sensitive PEC bioanalysis, which to our knowledge has not been reported. With the use of various other enzymes, this work could serve as a general basis for the PEC bioanalysis of numerous other target of interest. Image 1 • Peroxidase (HRP)-loaded liposomes were prepared. • The released HRP could induce enhanced biocatalytic precipitation. • The as-derived photoelectrochemical immunoassay has good performance. [ABSTRACT FROM AUTHOR]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:Liposome-assisted photoelectrochemical (PEC) bioanalysis represents one of the latest frontiers in the arena of PEC bioanalysis. This work reports a general enzyme-amplified liposomal PEC bioanalysis protocol via the use of enzyme-loaded liposomes to boost the biocatalytic precipitation (BCP) effect. In the representative system, the horseradish peroxidase (HRP)-loaded liposome (HRPLL) and the Au nanoclusters (NCs)/Au nanoparticles (NPs)/TiO 2 nanotubes (NTs) framework (AATF) were used as liposomal label and photoelectrode, respectively. In the detection, the sandwich immunocomplex reaction was accomplished in a 96-well plate to confine the HRPLL label, which was then lysed to release the HRP molecules to initiate the BCP process. Due to the amplified formation of HRP-induced BCP on the AATF scaffold, the photo-current response correlated closely with the immunorecognition process and the analyte could be detected very sensitively. This work features the first integration of enzyme-loaded liposomes and the BCP for sensitive PEC bioanalysis, which to our knowledge has not been reported. With the use of various other enzymes, this work could serve as a general basis for the PEC bioanalysis of numerous other target of interest. Image 1 • Peroxidase (HRP)-loaded liposomes were prepared. • The released HRP could induce enhanced biocatalytic precipitation. • The as-derived photoelectrochemical immunoassay has good performance. [ABSTRACT FROM AUTHOR]
ISSN:00032670
DOI:10.1016/j.aca.2020.04.025