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Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules.

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Title: Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules.
Authors: Andler, Rodrigo1 (AUTHOR) randler@ucm.cl, Valdés, Cristian2 (AUTHOR), Díaz-Barrera, Alvaro3 (AUTHOR), Steinbüchel, Alexander4,5 (AUTHOR)
Source: New Biotechnology. Sep2020, Vol. 58, p10-16. 7p.
Subjects: Electrospray ionization mass spectrometry, Polymer degradation, Solvent extraction, Organic solvents, Bioconversion
Abstract: • The enzyme Lcp1 VH2 is active in the presence of organic solvents. • Oligo(cis -1,4-isoprene) molecules continuously migrated to the organic phase. • Mass distribution of degradation products depended on the organic solvent. • Multi-phase systems can be used for continuous extraction of catalysis products. Biotechnological processes for the partial degradation or transformation of poly(cis -1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme 'latex clearing protein' (Lcp) to transform the polymer into more hydrophilic oligo-isoprenoids results in modifications of the rubber structure and the synthesis of new material. In order to find an alternative process to recover the degradation products, a continuous extraction method using a biphasic system is described. The enzymatic activity of Lcp1 VH2 was studied in the presence of ethyl acetate and pentane as extraction solvents. Oligo(cis -1,4-isoprene) molecular species were isolated from the organic phase and analyzed by Electrospray Ionization Mass Spectrometry. The enzymatic reaction process was evaluated in terms of the biotransformation yield of poly(cis -1,4-isoprene) rubber into the corresponding degradation products. Biotransformation yields of between 42–52 % were achieved depending on the enzymatic reactor design and the extraction solvent. The results also showed that the mass distribution of the oligo(cis -1,4-isoprene) depended on the organic solvent applied. A novel, simple and effective process is demonstrated for biotransformation of poly(cis -1,4-isoprene) rubber with high oligo-isoprenoid molecules recovery yields. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:• The enzyme Lcp1 VH2 is active in the presence of organic solvents. • Oligo(cis -1,4-isoprene) molecules continuously migrated to the organic phase. • Mass distribution of degradation products depended on the organic solvent. • Multi-phase systems can be used for continuous extraction of catalysis products. Biotechnological processes for the partial degradation or transformation of poly(cis -1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme 'latex clearing protein' (Lcp) to transform the polymer into more hydrophilic oligo-isoprenoids results in modifications of the rubber structure and the synthesis of new material. In order to find an alternative process to recover the degradation products, a continuous extraction method using a biphasic system is described. The enzymatic activity of Lcp1 VH2 was studied in the presence of ethyl acetate and pentane as extraction solvents. Oligo(cis -1,4-isoprene) molecular species were isolated from the organic phase and analyzed by Electrospray Ionization Mass Spectrometry. The enzymatic reaction process was evaluated in terms of the biotransformation yield of poly(cis -1,4-isoprene) rubber into the corresponding degradation products. Biotransformation yields of between 42–52 % were achieved depending on the enzymatic reactor design and the extraction solvent. The results also showed that the mass distribution of the oligo(cis -1,4-isoprene) depended on the organic solvent applied. A novel, simple and effective process is demonstrated for biotransformation of poly(cis -1,4-isoprene) rubber with high oligo-isoprenoid molecules recovery yields. [ABSTRACT FROM AUTHOR]
ISSN:18716784
DOI:10.1016/j.nbt.2020.05.001