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Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules.

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Title: Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules.
Authors: Andler, Rodrigo1 (AUTHOR) randler@ucm.cl, Valdés, Cristian2 (AUTHOR), Díaz-Barrera, Alvaro3 (AUTHOR), Steinbüchel, Alexander4,5 (AUTHOR)
Source: New Biotechnology. Sep2020, Vol. 58, p10-16. 7p.
Subjects: Electrospray ionization mass spectrometry, Polymer degradation, Solvent extraction, Organic solvents, Bioconversion
Abstract: • The enzyme Lcp1 VH2 is active in the presence of organic solvents. • Oligo(cis -1,4-isoprene) molecules continuously migrated to the organic phase. • Mass distribution of degradation products depended on the organic solvent. • Multi-phase systems can be used for continuous extraction of catalysis products. Biotechnological processes for the partial degradation or transformation of poly(cis -1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme 'latex clearing protein' (Lcp) to transform the polymer into more hydrophilic oligo-isoprenoids results in modifications of the rubber structure and the synthesis of new material. In order to find an alternative process to recover the degradation products, a continuous extraction method using a biphasic system is described. The enzymatic activity of Lcp1 VH2 was studied in the presence of ethyl acetate and pentane as extraction solvents. Oligo(cis -1,4-isoprene) molecular species were isolated from the organic phase and analyzed by Electrospray Ionization Mass Spectrometry. The enzymatic reaction process was evaluated in terms of the biotransformation yield of poly(cis -1,4-isoprene) rubber into the corresponding degradation products. Biotransformation yields of between 42–52 % were achieved depending on the enzymatic reactor design and the extraction solvent. The results also showed that the mass distribution of the oligo(cis -1,4-isoprene) depended on the organic solvent applied. A novel, simple and effective process is demonstrated for biotransformation of poly(cis -1,4-isoprene) rubber with high oligo-isoprenoid molecules recovery yields. [ABSTRACT FROM AUTHOR]
Copyright of New Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 144374051
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PubTypeId: academicJournal
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  Data: Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules.
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  Data: <searchLink fieldCode="AR" term="%22Andler%2C+Rodrigo%22">Andler, Rodrigo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> randler@ucm.cl</i><br /><searchLink fieldCode="AR" term="%22Valdés%2C+Cristian%22">Valdés, Cristian</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Díaz-Barrera%2C+Alvaro%22">Díaz-Barrera, Alvaro</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Steinbüchel%2C+Alexander%22">Steinbüchel, Alexander</searchLink><relatesTo>4,5</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22New+Biotechnology%22">New Biotechnology</searchLink>. Sep2020, Vol. 58, p10-16. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Electrospray+ionization+mass+spectrometry%22">Electrospray ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Polymer+degradation%22">Polymer degradation</searchLink><br /><searchLink fieldCode="DE" term="%22Solvent+extraction%22">Solvent extraction</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+solvents%22">Organic solvents</searchLink><br /><searchLink fieldCode="DE" term="%22Bioconversion%22">Bioconversion</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: • The enzyme Lcp1 VH2 is active in the presence of organic solvents. • Oligo(cis -1,4-isoprene) molecules continuously migrated to the organic phase. • Mass distribution of degradation products depended on the organic solvent. • Multi-phase systems can be used for continuous extraction of catalysis products. Biotechnological processes for the partial degradation or transformation of poly(cis -1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme 'latex clearing protein' (Lcp) to transform the polymer into more hydrophilic oligo-isoprenoids results in modifications of the rubber structure and the synthesis of new material. In order to find an alternative process to recover the degradation products, a continuous extraction method using a biphasic system is described. The enzymatic activity of Lcp1 VH2 was studied in the presence of ethyl acetate and pentane as extraction solvents. Oligo(cis -1,4-isoprene) molecular species were isolated from the organic phase and analyzed by Electrospray Ionization Mass Spectrometry. The enzymatic reaction process was evaluated in terms of the biotransformation yield of poly(cis -1,4-isoprene) rubber into the corresponding degradation products. Biotransformation yields of between 42–52 % were achieved depending on the enzymatic reactor design and the extraction solvent. The results also showed that the mass distribution of the oligo(cis -1,4-isoprene) depended on the organic solvent applied. A novel, simple and effective process is demonstrated for biotransformation of poly(cis -1,4-isoprene) rubber with high oligo-isoprenoid molecules recovery yields. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of New Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Text: English
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      – SubjectFull: Solvent extraction
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      – TitleFull: Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules.
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              Text: Sep2020
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