View in EDS

Two-photon active fluorescent indicator for detecting NADH dynamics in live cells and tumor tissue.

Saved in:
Bibliographic Details
Title: Two-photon active fluorescent indicator for detecting NADH dynamics in live cells and tumor tissue.
Authors: Podder, Arup1 (AUTHOR), Thirumalaivasan, Natesan1,2 (AUTHOR), Chao, Yu Kai2 (AUTHOR), Kukutla, Prasanna1 (AUTHOR), Wu, Shu-Pao1,2 (AUTHOR) spwu@mail.nctu.edu.tw, Bhuniya, Sankarprasad1,3 (AUTHOR) b_sankarprasad@cb.amrita.edu
Source: Sensors & Actuators B: Chemical. Dec2020, Vol. 324, pN.PAG-N.PAG. 1p.
Subjects: Colon tumors, Fluorescent probes, Reduction potential, Crosstalk, Oxidative phosphorylation, Oxidation-reduction reaction, Cofactors (Biochemistry)
Abstract: • The first example of NADH responsive two-photon fluorescent probe. • Only it showed the emission signals at 460 nm upon two photon excitations at 860 nm in the presence of NADH/NAD(P)H. • We established cross talk between ROS regulator GSH. • It visualized NADH dependent mitochondrial oxidative phosphorylation cycle. • The probe selectively levelled tumor tissue based on overexpressed NADH. We have developed a two-photon fluorescent indicator (MC 2P) that participates in a redox reaction with NADH to provide a fluorescence signal at 460 nm. The quantum yield of the indicator MC 2P has increased about 26-fold in the presence of NADH. High two-photon efficiency (GM = 80.94) of the indicator MC 2P has considered it as a unique device for assessing NADH expression in live cells without interference from native fluorogenic entities. It rapidly internalized into live cells and showed a considerable fluorescence signal in cancer cells than the immune cells (RAW 264.7). The indicator MC 2P has provided information on the fluctuation of NADH levels depending on the variation of metabolites in the glycolysis pathway. Further, we noticed an increment of NADH level in mitochondrial respiratory cascade by inhibiting NADH-ubiquinone oxo reductase (complex I) using rotenone. On the contrary, uncoupler (CCCP) induced depletion of NADH. The dynamic expression of NAD(P)H has increased for counterbalancing mitochondrial redox potential during ROS (H 2 O 2) suppression by GSH. Finally, in an vivo experiment, the indicator MC 2P has distinguished colon tumor (HT-29 cells) tissue from healthy tissue by providing two-photon fluorescence images. [ABSTRACT FROM AUTHOR]
Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:• The first example of NADH responsive two-photon fluorescent probe. • Only it showed the emission signals at 460 nm upon two photon excitations at 860 nm in the presence of NADH/NAD(P)H. • We established cross talk between ROS regulator GSH. • It visualized NADH dependent mitochondrial oxidative phosphorylation cycle. • The probe selectively levelled tumor tissue based on overexpressed NADH. We have developed a two-photon fluorescent indicator (MC 2P) that participates in a redox reaction with NADH to provide a fluorescence signal at 460 nm. The quantum yield of the indicator MC 2P has increased about 26-fold in the presence of NADH. High two-photon efficiency (GM = 80.94) of the indicator MC 2P has considered it as a unique device for assessing NADH expression in live cells without interference from native fluorogenic entities. It rapidly internalized into live cells and showed a considerable fluorescence signal in cancer cells than the immune cells (RAW 264.7). The indicator MC 2P has provided information on the fluctuation of NADH levels depending on the variation of metabolites in the glycolysis pathway. Further, we noticed an increment of NADH level in mitochondrial respiratory cascade by inhibiting NADH-ubiquinone oxo reductase (complex I) using rotenone. On the contrary, uncoupler (CCCP) induced depletion of NADH. The dynamic expression of NAD(P)H has increased for counterbalancing mitochondrial redox potential during ROS (H 2 O 2) suppression by GSH. Finally, in an vivo experiment, the indicator MC 2P has distinguished colon tumor (HT-29 cells) tissue from healthy tissue by providing two-photon fluorescence images. [ABSTRACT FROM AUTHOR]
ISSN:09254005
DOI:10.1016/j.snb.2020.128637