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Tandem surface-induced dissociation of protein complexes on an ultrahigh resolution platform.

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Title: Tandem surface-induced dissociation of protein complexes on an ultrahigh resolution platform.
Authors: Snyder, Dalton T.1 (AUTHOR), Lin, Yu-Fu1,2 (AUTHOR), Somogyi, Arpad1 (AUTHOR), Wysocki, Vicki H.1,2 (AUTHOR) wysocki.11@osu.edu
Source: International Journal of Mass Spectrometry. Mar2021, Vol. 461, pN.PAG-N.PAG. 1p.
Subjects: Cyclotron resonance, Daughter ions, Mass spectrometers, Proteins, Fourier transforms
Abstract: We describe instrumentation for conducting tandem surface-induced dissociation (tSID) of native protein complexes on an ultrahigh resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The two stages of SID are accomplished with split lenses replacing the entrance lenses of the quadrupole mass filter (stage 1, referred to herein as SID-Q) and the collision cell (stage 2, Q-SID). After SID-Q, the scattered projectile ions and subcomplexes formed in transit traverse the pre-filter prior to the mass-selecting quadrupole, providing preliminary insights into the SID fragmentation kinetics of noncovalent protein complexes. The isolated SID fragments (subcomplexes) are then fragmented by SID in the collision cell entrance lens (Q-SID), generating subcomplexes of subcomplexes. We show that the ultrahigh resolution of the FT-ICR can be used for deconvolving species overlapping in m/z, which are particularly prominent in tandem SID spectra due to the combination of symmetric charge partitioning and narrow product ion charge state distributions. Various protein complex topologies are explored, including homotetramers, homopentamers, a homohexamer, and a heterohexamer. Image 1 • Tandem SID designs are described for FT-ICR and Q-IM-TOF platforms. • Tandem SID of protein complexes reveals subunit connectivity. • Ultrahigh resolution FT-ICR deconvolves overlapping charge states. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:We describe instrumentation for conducting tandem surface-induced dissociation (tSID) of native protein complexes on an ultrahigh resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The two stages of SID are accomplished with split lenses replacing the entrance lenses of the quadrupole mass filter (stage 1, referred to herein as SID-Q) and the collision cell (stage 2, Q-SID). After SID-Q, the scattered projectile ions and subcomplexes formed in transit traverse the pre-filter prior to the mass-selecting quadrupole, providing preliminary insights into the SID fragmentation kinetics of noncovalent protein complexes. The isolated SID fragments (subcomplexes) are then fragmented by SID in the collision cell entrance lens (Q-SID), generating subcomplexes of subcomplexes. We show that the ultrahigh resolution of the FT-ICR can be used for deconvolving species overlapping in m/z, which are particularly prominent in tandem SID spectra due to the combination of symmetric charge partitioning and narrow product ion charge state distributions. Various protein complex topologies are explored, including homotetramers, homopentamers, a homohexamer, and a heterohexamer. Image 1 • Tandem SID designs are described for FT-ICR and Q-IM-TOF platforms. • Tandem SID of protein complexes reveals subunit connectivity. • Ultrahigh resolution FT-ICR deconvolves overlapping charge states. [ABSTRACT FROM AUTHOR]
ISSN:13873806
DOI:10.1016/j.ijms.2020.116503