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Solid-state culture of Azospirillum brasilense: a reliable technology for biofertilizer production from laboratory to pilot scale.

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Title: Solid-state culture of Azospirillum brasilense: a reliable technology for biofertilizer production from laboratory to pilot scale.
Authors: Martínez-Ramírez, C.1 (AUTHOR), Esquivel-Cote, R.2 (AUTHOR), Ferrera-Cerrato, R.2 (AUTHOR), Martínez-Ruiz, J. A.1 (AUTHOR), Rodríguez-Serrano, G.1 (AUTHOR), Saucedo-Castañeda, G.1 (AUTHOR) saucedo@xanum.uam.mx
Source: Bioprocess & Biosystems Engineering. Jul2021, Vol. 44 Issue 7, p1525-1538. 14p.
Subjects: Azospirillum brasilense, Dimensionless numbers, Biofertilizers, Cell survival, Laboratories, Mathematical models
Abstract: A biofertilizer of Azospirillum brasilense was produced in solid-state culture (SSC) from laboratory to pilot scale. Similar operation conditions (continuous aeration and mild intermittent mixing) and two dimensionless numbers with similar L/D ratio and a similar working volume were applied to reach a scale-up factor of 75. An innovative bioreactor with rotating helical ribbons (15 kg wet matter) was used at pilot scale. A mathematical model was proposed and validated to evaluate the respirometry trends at laboratory and pilot scale exhibiting similar behavior. The cell viability was (1.3 ± 0.4) × 109 and (1.3 ± 0.3) × 109 colony-forming units per gram of initial dry mass at laboratory and pilot scale, at 36 and 43 h, respectively. A. brasilense maintains its viability twelve months of storage at 4 and 30 °C. This is the first report of A. brasilense being cultivated in SSC under controlled conditions. SSC processes involving unicellular microorganisms with tolerance to agitation are a promising technology to produce biofertilizers. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:A biofertilizer of Azospirillum brasilense was produced in solid-state culture (SSC) from laboratory to pilot scale. Similar operation conditions (continuous aeration and mild intermittent mixing) and two dimensionless numbers with similar L/D ratio and a similar working volume were applied to reach a scale-up factor of 75. An innovative bioreactor with rotating helical ribbons (15 kg wet matter) was used at pilot scale. A mathematical model was proposed and validated to evaluate the respirometry trends at laboratory and pilot scale exhibiting similar behavior. The cell viability was (1.3 ± 0.4) × 109 and (1.3 ± 0.3) × 109 colony-forming units per gram of initial dry mass at laboratory and pilot scale, at 36 and 43 h, respectively. A. brasilense maintains its viability twelve months of storage at 4 and 30 °C. This is the first report of A. brasilense being cultivated in SSC under controlled conditions. SSC processes involving unicellular microorganisms with tolerance to agitation are a promising technology to produce biofertilizers. [ABSTRACT FROM AUTHOR]
ISSN:16157591
DOI:10.1007/s00449-021-02537-3