View in EDS

Photothermal lateral flow immunoassay using microfiber long-period grating.

Saved in:
Bibliographic Details
Title: Photothermal lateral flow immunoassay using microfiber long-period grating.
Authors: Huang, Tiansheng1 (AUTHOR), Fu, Qiangqiang1,2 (AUTHOR), Sun, Li-Peng1 (AUTHOR) lpsun@jnu.edu.cn, Liu, Peiyuan1 (AUTHOR), Wu, Ze3 (AUTHOR), Li, Kaqiang1 (AUTHOR), Xiao, Ruitao1 (AUTHOR), Yang, Xiao1 (AUTHOR), Huang, Yan1 (AUTHOR), Lin, Wenfu1 (AUTHOR), Lu, Hanglin1 (AUTHOR), Ma, Jun1 (AUTHOR), Wang, Wei1 (AUTHOR), Li, Jie1 (AUTHOR), Tang, Yong1,4 (AUTHOR) tyjaq7926@163.com, Guan, Bai-Ou1 (AUTHOR) tguanbo@jnu.edu.cn
Source: Sensors & Actuators B: Chemical. Oct2021, Vol. 344, pN.PAG-N.PAG. 1p.
Subjects: Electrochemiluminescence, Immunoassay, Refractive index, Gold nanoparticles, Wave functions, Detection limit
Abstract: • A new hybrid biosensor based on fiber-optic sensor and papered-based biosensor. • Microfiber long period grating combined with thermal-optic polymer material for efficient acquiring photothermal signal. • Plasmonic nanoparticle used in lateral flow immunoassay for photothermal generation and as immuno-probe. • High sensitivity due to the structure of strong evanescent wave and the cascade strategy of thermo-optic transduction. • Quantitative analysis with low LOD of 0.219 ng/mL, wide working range (0.195–200 ng/mL), and high selectivity for Cr3+. Paper-based biosensors are important analytical tools in biological and chemical fields. To increase the detection limit and range, here we demonstrated a microfiber long-period grating (mLPG) based biosensor for lateral flow immunoassay (mLPG-LFIA). Compared to conventional paper-based biosensor, the mLPG with strong evanescent wave functions as an ultrasensitive tool for detecting the refractive index changes induced by the localized plasmonic heating of antibody-conjugated gold nanoparticles. The LFIA concentration level is encoded into the refractive index modulation and can be subsequently read by measuring the spectral shift of the mLPG. As proof-of-concept demonstration, quantitative analysis of heavy-metal chromium-ion concentrations ranging from of 0.195 to 200 ng/mL is performed, and a detection limit of 0.219 ng/mL is achieved. The proposed technique is sensitive, easy-to-use, and can serve as a powerful tool to analyze trace chemicals or biochemicals for application in the area of medicine, foods safety and environment monitoring. [ABSTRACT FROM AUTHOR]
Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:• A new hybrid biosensor based on fiber-optic sensor and papered-based biosensor. • Microfiber long period grating combined with thermal-optic polymer material for efficient acquiring photothermal signal. • Plasmonic nanoparticle used in lateral flow immunoassay for photothermal generation and as immuno-probe. • High sensitivity due to the structure of strong evanescent wave and the cascade strategy of thermo-optic transduction. • Quantitative analysis with low LOD of 0.219 ng/mL, wide working range (0.195–200 ng/mL), and high selectivity for Cr3+. Paper-based biosensors are important analytical tools in biological and chemical fields. To increase the detection limit and range, here we demonstrated a microfiber long-period grating (mLPG) based biosensor for lateral flow immunoassay (mLPG-LFIA). Compared to conventional paper-based biosensor, the mLPG with strong evanescent wave functions as an ultrasensitive tool for detecting the refractive index changes induced by the localized plasmonic heating of antibody-conjugated gold nanoparticles. The LFIA concentration level is encoded into the refractive index modulation and can be subsequently read by measuring the spectral shift of the mLPG. As proof-of-concept demonstration, quantitative analysis of heavy-metal chromium-ion concentrations ranging from of 0.195 to 200 ng/mL is performed, and a detection limit of 0.219 ng/mL is achieved. The proposed technique is sensitive, easy-to-use, and can serve as a powerful tool to analyze trace chemicals or biochemicals for application in the area of medicine, foods safety and environment monitoring. [ABSTRACT FROM AUTHOR]
ISSN:09254005
DOI:10.1016/j.snb.2021.130283