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Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry.

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Title: Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry.
Authors: Islam, Syful1,2 (AUTHOR), Alam, Rafiqul1 (AUTHOR), Kim, Sunghwan1,3 (AUTHOR) sunghwank@knu.ac.kr
Source: Food Chemistry. Mar2022:Part B, Vol. 373, pN.PAG-N.PAG. 1p.
Subjects: Electrospray ionization mass spectrometry, Plant metabolites, Matrix-assisted laser desorption-ionization, Mass spectrometry, Chemical formulas, Centella asiatica, Cyclotrons, Desorption
Abstract: [Display omitted] • LDI FT-ICR MS enhanced the metabolite profiling of Centella asiatica. • No interference peaks were observed for double-sided tape in LDI spectra. • LDI detected both solvent extracted and non-extracted compounds. • Small amount of plant sample (∼200 µg) was consumed in LDI techniques. In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:[Display omitted] • LDI FT-ICR MS enhanced the metabolite profiling of Centella asiatica. • No interference peaks were observed for double-sided tape in LDI spectra. • LDI detected both solvent extracted and non-extracted compounds. • Small amount of plant sample (∼200 µg) was consumed in LDI techniques. In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase. [ABSTRACT FROM AUTHOR]
ISSN:03088146
DOI:10.1016/j.foodchem.2021.131541