Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry.
Saved in:
| Title: | Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry. |
|---|---|
| Authors: | Islam, Syful1,2 (AUTHOR), Alam, Rafiqul1 (AUTHOR), Kim, Sunghwan1,3 (AUTHOR) sunghwank@knu.ac.kr |
| Source: | Food Chemistry. Mar2022:Part B, Vol. 373, pN.PAG-N.PAG. 1p. |
| Subjects: | Electrospray ionization mass spectrometry, Plant metabolites, Matrix-assisted laser desorption-ionization, Mass spectrometry, Chemical formulas, Centella asiatica, Cyclotrons, Desorption |
| Abstract: | [Display omitted] • LDI FT-ICR MS enhanced the metabolite profiling of Centella asiatica. • No interference peaks were observed for double-sided tape in LDI spectra. • LDI detected both solvent extracted and non-extracted compounds. • Small amount of plant sample (∼200 µg) was consumed in LDI techniques. In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase. [ABSTRACT FROM AUTHOR] |
| Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 153978069 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Islam%2C+Syful%22">Islam, Syful</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alam%2C+Rafiqul%22">Alam, Rafiqul</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim%2C+Sunghwan%22">Kim, Sunghwan</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> sunghwank@knu.ac.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Food+Chemistry%22">Food Chemistry</searchLink>. Mar2022:Part B, Vol. 373, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Electrospray+ionization+mass+spectrometry%22">Electrospray ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+metabolites%22">Plant metabolites</searchLink><br /><searchLink fieldCode="DE" term="%22Matrix-assisted+laser+desorption-ionization%22">Matrix-assisted laser desorption-ionization</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+formulas%22">Chemical formulas</searchLink><br /><searchLink fieldCode="DE" term="%22Centella+asiatica%22">Centella asiatica</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclotrons%22">Cyclotrons</searchLink><br /><searchLink fieldCode="DE" term="%22Desorption%22">Desorption</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: [Display omitted] • LDI FT-ICR MS enhanced the metabolite profiling of Centella asiatica. • No interference peaks were observed for double-sided tape in LDI spectra. • LDI detected both solvent extracted and non-extracted compounds. • Small amount of plant sample (∼200 µg) was consumed in LDI techniques. In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=153978069 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.foodchem.2021.131541 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Electrospray ionization mass spectrometry Type: general – SubjectFull: Plant metabolites Type: general – SubjectFull: Matrix-assisted laser desorption-ionization Type: general – SubjectFull: Mass spectrometry Type: general – SubjectFull: Chemical formulas Type: general – SubjectFull: Centella asiatica Type: general – SubjectFull: Cyclotrons Type: general – SubjectFull: Desorption Type: general Titles: – TitleFull: Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion cyclotron mass spectrometry. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Islam, Syful – PersonEntity: Name: NameFull: Alam, Rafiqul – PersonEntity: Name: NameFull: Kim, Sunghwan IsPartOfRelationships: – BibEntity: Dates: – D: 31 M: 03 Text: Mar2022:Part B Type: published Y: 2022 Identifiers: – Type: issn-print Value: 03088146 Numbering: – Type: volume Value: 373 Titles: – TitleFull: Food Chemistry Type: main |
| ResultId | 1 |
