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Introgression molecular analysis of a leaf rust resistance gene fromCoffea libericaintoC. arabicaL.

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Title: Introgression molecular analysis of a leaf rust resistance gene fromCoffea libericaintoC. arabicaL.
Authors: Prakash, N. S.1, Marques, D. V.2, Varzea, V. M. P.2, Silva, M. C.2, Combes, M. C.3, Lashermes, P.3 Philippe.Lashermes@mpl.ird.fr
Source: Theoretical & Applied Genetics. Oct2004, Vol. 109 Issue 6, p1311-1317. 7p.
Subjects: Plant diseases, Coffee industry, Genetic polymorphisms, Rubiaceae, Genetics, Gentianales
Abstract: Leaf rust caused by the fungusHemileia vastatrixis the most devastating disease of arabica coffee (Coffea arabica). Therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. The purpose of the present work was to gain insight into the mechanism of introgression intoC. arabicaof a leaf rust resistance gene fromC. liberica(i.e. SH3 resistance factor) and to identify associated molecular markers. An F2 progeny (i.e. 101 individuals) derived from a cross between Matari, an arabica accession and liberica-introgressed line S.288, was evaluated for resistance against three different races ofH. vastatrix. The progeny segregated for the SH3 gene in a 3:1 ratio, as expected for a single dominant gene. Amplified fragment length polymorphism analysis of a population subset using 80 different primer combinations revealed that at least half of the total polymorphism observed in the population is associated with introgression ofC. libericachromosome fragments. Furthermore, 15 primer combinations generating candidate marker bands associated with the SH3 resistance gene were used to analyse the whole F2 population. A total of 34 marker bands originating from S.288 and attributable to introgression were scored. None exhibited segregation distortion. Linkage analysis revealed only three distinct introgressed fragments corresponding to a total length of 52.8 cM. Twenty-one markers were strongly associated (LOD score>14) with the SH3 gene and were grouped together in a single linkage group of 6.3 cM. The results are discussed in relation to the efficient use of genetic resources in arabica breeding. [ABSTRACT FROM AUTHOR]
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Abstract:Leaf rust caused by the fungusHemileia vastatrixis the most devastating disease of arabica coffee (Coffea arabica). Therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. The purpose of the present work was to gain insight into the mechanism of introgression intoC. arabicaof a leaf rust resistance gene fromC. liberica(i.e. SH3 resistance factor) and to identify associated molecular markers. An F2 progeny (i.e. 101 individuals) derived from a cross between Matari, an arabica accession and liberica-introgressed line S.288, was evaluated for resistance against three different races ofH. vastatrix. The progeny segregated for the SH3 gene in a 3:1 ratio, as expected for a single dominant gene. Amplified fragment length polymorphism analysis of a population subset using 80 different primer combinations revealed that at least half of the total polymorphism observed in the population is associated with introgression ofC. libericachromosome fragments. Furthermore, 15 primer combinations generating candidate marker bands associated with the SH3 resistance gene were used to analyse the whole F2 population. A total of 34 marker bands originating from S.288 and attributable to introgression were scored. None exhibited segregation distortion. Linkage analysis revealed only three distinct introgressed fragments corresponding to a total length of 52.8 cM. Twenty-one markers were strongly associated (LOD score>14) with the SH3 gene and were grouped together in a single linkage group of 6.3 cM. The results are discussed in relation to the efficient use of genetic resources in arabica breeding. [ABSTRACT FROM AUTHOR]
ISSN:00405752
DOI:10.1007/s00122-004-1748-z