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Monoubiquitinated Histone H1B Is Required for Antiviral Protection in CD41T Cells Resistant to HIV-1.

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Bibliographic Details
Title:	Monoubiquitinated Histone H1B Is Required for Antiviral Protection in CD4¹T Cells Resistant to HIV-1.
Authors:	Lesner, Adam¹, Kartvelishvili, Alex¹, Lesniak, Jakob², Nikolov, Dimitar², Kartvelishvili, Magdalena¹, Trillo-Pazos, Gusta¹, Zablotna, Ewa¹, Simm, Malgorzata¹ ms130@columbia.edu
Source:	Biochemistry. 12/28/2004, Vol. 43 Issue 51, p16203-16211. 9p.
Subjects:	T cells, HIV, Histones, Cell culture, HTLV, Cultures (Biology)
Abstract:	Linker histone HIB (HIB) coeluted with an antiviral activity during the purification of HIV-1 resistance factor (HRF) from supernatants of HRF(+) cells. Western blot analysis of the supernatant using α-Hi and α-ubiquitin antibodies detected the same band of roughly 46 kDa; this band was absent from the control supematant. Depletion of histone from biologically active material did not affect its potential, suggesting that ubiquitinated HIB is not required for the HRF-mediated antiviral protection in HIV-1 susceptible target cells; however, specific silencing of histone HIB via RNAi in HRF(+) cells reduced the biological activity of cell culture supematants by 96% and reversed the HIV-l resistance phenotype of HRF(+) cells. Exposure to HRF induced ubiquitination and secretion of HIB from target HIV-l susceptible cells, suggesting that ubiquitinated HIB is a cofactor of HRF, possibly regulating its expression and secretion from CD4+T cells induced to resist HIV-i infection. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	Linker histone HIB (HIB) coeluted with an antiviral activity during the purification of HIV-1 resistance factor (HRF) from supernatants of HRF(+) cells. Western blot analysis of the supernatant using α-Hi and α-ubiquitin antibodies detected the same band of roughly 46 kDa; this band was absent from the control supematant. Depletion of histone from biologically active material did not affect its potential, suggesting that ubiquitinated HIB is not required for the HRF-mediated antiviral protection in HIV-1 susceptible target cells; however, specific silencing of histone HIB via RNAi in HRF(+) cells reduced the biological activity of cell culture supematants by 96% and reversed the HIV-l resistance phenotype of HRF(+) cells. Exposure to HRF induced ubiquitination and secretion of HIB from target HIV-l susceptible cells, suggesting that ubiquitinated HIB is a cofactor of HRF, possibly regulating its expression and secretion from CD4+T cells induced to resist HIV-i infection. [ABSTRACT FROM AUTHOR]
ISSN:	00062960
DOI:	10.1021/bi0492758