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Monoubiquitinated Histone H1B Is Required for Antiviral Protection in CD41T Cells Resistant to HIV-1.

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Title: Monoubiquitinated Histone H1B Is Required for Antiviral Protection in CD41T Cells Resistant to HIV-1.
Authors: Lesner, Adam1, Kartvelishvili, Alex1, Lesniak, Jakob2, Nikolov, Dimitar2, Kartvelishvili, Magdalena1, Trillo-Pazos, Gusta1, Zablotna, Ewa1, Simm, Malgorzata1 ms130@columbia.edu
Source: Biochemistry. 12/28/2004, Vol. 43 Issue 51, p16203-16211. 9p.
Subjects: T cells, HIV, Histones, Cell culture, HTLV, Cultures (Biology)
Abstract: Linker histone HIB (HIB) coeluted with an antiviral activity during the purification of HIV-1 resistance factor (HRF) from supernatants of HRF(+) cells. Western blot analysis of the supernatant using α-Hi and α-ubiquitin antibodies detected the same band of roughly 46 kDa; this band was absent from the control supematant. Depletion of histone from biologically active material did not affect its potential, suggesting that ubiquitinated HIB is not required for the HRF-mediated antiviral protection in HIV-1 susceptible target cells; however, specific silencing of histone HIB via RNAi in HRF(+) cells reduced the biological activity of cell culture supematants by 96% and reversed the HIV-l resistance phenotype of HRF(+) cells. Exposure to HRF induced ubiquitination and secretion of HIB from target HIV-l susceptible cells, suggesting that ubiquitinated HIB is a cofactor of HRF, possibly regulating its expression and secretion from CD4+T cells induced to resist HIV-i infection. [ABSTRACT FROM AUTHOR]
Copyright of Biochemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 15659017
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Monoubiquitinated Histone H1B Is Required for Antiviral Protection in CD4<superscript>1</superscript>T Cells Resistant to HIV-1.
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  Data: <searchLink fieldCode="AR" term="%22Lesner%2C+Adam%22">Lesner, Adam</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kartvelishvili%2C+Alex%22">Kartvelishvili, Alex</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lesniak%2C+Jakob%22">Lesniak, Jakob</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Nikolov%2C+Dimitar%22">Nikolov, Dimitar</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kartvelishvili%2C+Magdalena%22">Kartvelishvili, Magdalena</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Trillo-Pazos%2C+Gusta%22">Trillo-Pazos, Gusta</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zablotna%2C+Ewa%22">Zablotna, Ewa</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Simm%2C+Malgorzata%22">Simm, Malgorzata</searchLink><relatesTo>1</relatesTo><i> ms130@columbia.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Biochemistry%22">Biochemistry</searchLink>. 12/28/2004, Vol. 43 Issue 51, p16203-16211. 9p.
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  Data: <searchLink fieldCode="DE" term="%22T+cells%22">T cells</searchLink><br /><searchLink fieldCode="DE" term="%22HIV%22">HIV</searchLink><br /><searchLink fieldCode="DE" term="%22Histones%22">Histones</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+culture%22">Cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22HTLV%22">HTLV</searchLink><br /><searchLink fieldCode="DE" term="%22Cultures+%28Biology%29%22">Cultures (Biology)</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Linker histone HIB (HIB) coeluted with an antiviral activity during the purification of HIV-1 resistance factor (HRF) from supernatants of HRF(+) cells. Western blot analysis of the supernatant using α-Hi and α-ubiquitin antibodies detected the same band of roughly 46 kDa; this band was absent from the control supematant. Depletion of histone from biologically active material did not affect its potential, suggesting that ubiquitinated HIB is not required for the HRF-mediated antiviral protection in HIV-1 susceptible target cells; however, specific silencing of histone HIB via RNAi in HRF(+) cells reduced the biological activity of cell culture supematants by 96% and reversed the HIV-l resistance phenotype of HRF(+) cells. Exposure to HRF induced ubiquitination and secretion of HIB from target HIV-l susceptible cells, suggesting that ubiquitinated HIB is a cofactor of HRF, possibly regulating its expression and secretion from CD4+T cells induced to resist HIV-i infection. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Biochemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1021/bi0492758
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        Text: English
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      – SubjectFull: Histones
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      – SubjectFull: Cell culture
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      – SubjectFull: HTLV
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      – SubjectFull: Cultures (Biology)
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      – TitleFull: Monoubiquitinated Histone H1B Is Required for Antiviral Protection in CD41T Cells Resistant to HIV-1.
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              Text: 12/28/2004
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              Y: 2004
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