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Through the looking-glass - Recent developments in reflectometry open new possibilities for biosensor applications.

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Title: Through the looking-glass - Recent developments in reflectometry open new possibilities for biosensor applications.
Authors: Fechner, Peter1 (AUTHOR) peter.fechner@uni-tuebingen.de, Gauglitz, Günter1 (AUTHOR), Proll, Günther1 (AUTHOR)
Source: Trends in Analytical Chemistry: TRAC. Nov2022, Vol. 156, pN.PAG-N.PAG. 1p.
Subjects: Reflectometry, Biosensors, Spectral reflectance, Art techniques, Spectral imaging, Interferometry
Abstract: Label-free biosensing has developed from niche application to state of the art biosensing technique throughout the past decades. The reason for the raise of acceptance for this technology is the fact, that it generates valuable kinetic data (on- and off-rates), does not require complicated sample-pre-treatment, and can be used in direct test formats. As the acceptance increased, label-free advanced and many different approaches with the same underlying principles have evolved. This review will take a brief look at the history, and explore the reflectometry family tree, which includes ellipsometry as the main ancestor, Reflectometric Interference Spectroscopy (RIfS), Biolayer Interferometry (BLI), Total Reflectometric Interference Spectroscopy (TRIS), Spectral Reflectance Imaging Biosensor (SRIB or also IRIS), 1ʎ Reflectometry, Arrayed Imaging Reflectometry (AIR), and Oblique-Incidence Reflectivity Difference microscopy (OI-RD) and highlight extraordinary examples of recent developments and their impact in biomolecular interaction analysis. • Reflectometric biosensing has evolved over the years. • High throughput is one of the key advantages of reflectometry compared the refractometry. • Complex biological questions can be answered with reflectometry. • New materials enhance the power of reflectometry. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Label-free biosensing has developed from niche application to state of the art biosensing technique throughout the past decades. The reason for the raise of acceptance for this technology is the fact, that it generates valuable kinetic data (on- and off-rates), does not require complicated sample-pre-treatment, and can be used in direct test formats. As the acceptance increased, label-free advanced and many different approaches with the same underlying principles have evolved. This review will take a brief look at the history, and explore the reflectometry family tree, which includes ellipsometry as the main ancestor, Reflectometric Interference Spectroscopy (RIfS), Biolayer Interferometry (BLI), Total Reflectometric Interference Spectroscopy (TRIS), Spectral Reflectance Imaging Biosensor (SRIB or also IRIS), 1ʎ Reflectometry, Arrayed Imaging Reflectometry (AIR), and Oblique-Incidence Reflectivity Difference microscopy (OI-RD) and highlight extraordinary examples of recent developments and their impact in biomolecular interaction analysis. • Reflectometric biosensing has evolved over the years. • High throughput is one of the key advantages of reflectometry compared the refractometry. • Complex biological questions can be answered with reflectometry. • New materials enhance the power of reflectometry. [ABSTRACT FROM AUTHOR]
ISSN:01659936
DOI:10.1016/j.trac.2022.116708