Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles.
Saved in:
| Title: | Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles. |
|---|---|
| Authors: | Jaapar, F. Nadhirah1 (AUTHOR), Parmin, N.A.1 (AUTHOR) azizahparmin@unimap.edu.my, Halim, N. Hamidah A1 (AUTHOR), Hashim, Uda1 (AUTHOR), Gopinath, Subash C.B.1,2 (AUTHOR), Halim, F. Syakirah1 (AUTHOR), Ruslinda, A. Rahim1 (AUTHOR), Voon, C.H.1 (AUTHOR), Uda, M.N.A.1 (AUTHOR), Uda, M.N.Afnan1 (AUTHOR), Nadzirah, Sh.3 (AUTHOR), Rejali, Zulida4 (AUTHOR), Afzan, Amilia4 (AUTHOR), Zakaria, Iffah Izzati5 (AUTHOR) |
| Source: | Biotechnology & Applied Biochemistry. Oct2022, Vol. 69 Issue 5, p1966-1983. 18p. |
| Subjects: | DNA probes, Gold nanoparticles, DNA viruses, Papillomaviruses, Pap test |
| Abstract: | The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single‐stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high‐end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross‐validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH‐GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH‐GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini‐size diagnostic devices for cervical cancer detection. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| Abstract: | The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single‐stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high‐end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross‐validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH‐GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH‐GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini‐size diagnostic devices for cervical cancer detection. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 08854513 |
| DOI: | 10.1002/bab.2260 |
