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Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography

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Title: Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography
Authors: Verweij-van Wissen, C.P.W.G.M.1,2 C.Verweij@akf.umcn.nl, Aarnoutse, R.E.1,2, Burger, D.M.1,2
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Feb2005, Vol. 816 Issue 1/2, p121-129. 9p.
Subjects: High performance liquid chromatography, Solid-phase analysis, Ultraviolet radiation, Nucleosides, Blood plasma
Abstract: Abstract: A reversed phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors (NRTIs) lamivudine, didanosine, stavudine, zidovudine and abacavir in plasma. The method involved solid-phase extraction with Oasis MAX cartridges from plasma, followed by high performance liquid chromatography with a SymmetryShield RP 18 column and ultraviolet detection set at a wavelength of 260nm. The assay was validated over the concentration range of 0.015–5mg/l for all five NRTIs. The average accuracies for the assay were 92–102%, inter- and intra-day coefficients of variation (CV) were <2.5% and extraction recoveries were higher than 97%. This method proved to be simple, accurate and precise, and is currently in use in our laboratory for the quantitative analysis of NRTIs in plasma. [Copyright &y& Elsevier]
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Database: Engineering Source
Description
Abstract:Abstract: A reversed phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors (NRTIs) lamivudine, didanosine, stavudine, zidovudine and abacavir in plasma. The method involved solid-phase extraction with Oasis MAX cartridges from plasma, followed by high performance liquid chromatography with a SymmetryShield RP 18 column and ultraviolet detection set at a wavelength of 260nm. The assay was validated over the concentration range of 0.015–5mg/l for all five NRTIs. The average accuracies for the assay were 92–102%, inter- and intra-day coefficients of variation (CV) were <2.5% and extraction recoveries were higher than 97%. This method proved to be simple, accurate and precise, and is currently in use in our laboratory for the quantitative analysis of NRTIs in plasma. [Copyright &y& Elsevier]
ISSN:15700232
DOI:10.1016/j.jchromb.2004.11.019