Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography
Saved in:
| Title: | Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography |
|---|---|
| Authors: | Verweij-van Wissen, C.P.W.G.M.1,2 C.Verweij@akf.umcn.nl, Aarnoutse, R.E.1,2, Burger, D.M.1,2 |
| Source: | Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Feb2005, Vol. 816 Issue 1/2, p121-129. 9p. |
| Subjects: | High performance liquid chromatography, Solid-phase analysis, Ultraviolet radiation, Nucleosides, Blood plasma |
| Abstract: | Abstract: A reversed phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors (NRTIs) lamivudine, didanosine, stavudine, zidovudine and abacavir in plasma. The method involved solid-phase extraction with Oasis MAX cartridges from plasma, followed by high performance liquid chromatography with a SymmetryShield RP 18 column and ultraviolet detection set at a wavelength of 260nm. The assay was validated over the concentration range of 0.015–5mg/l for all five NRTIs. The average accuracies for the assay were 92–102%, inter- and intra-day coefficients of variation (CV) were <2.5% and extraction recoveries were higher than 97%. This method proved to be simple, accurate and precise, and is currently in use in our laboratory for the quantitative analysis of NRTIs in plasma. [Copyright &y& Elsevier] |
| Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 16134182 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Verweij-van+Wissen%2C+C%2EP%2EW%2EG%2EM%2E%22">Verweij-van Wissen, C.P.W.G.M.</searchLink><relatesTo>1,2</relatesTo><i> C.Verweij@akf.umcn.nl</i><br /><searchLink fieldCode="AR" term="%22Aarnoutse%2C+R%2EE%2E%22">Aarnoutse, R.E.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Burger%2C+D%2EM%2E%22">Burger, D.M.</searchLink><relatesTo>1,2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Feb2005, Vol. 816 Issue 1/2, p121-129. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Solid-phase+analysis%22">Solid-phase analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Ultraviolet+radiation%22">Ultraviolet radiation</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleosides%22">Nucleosides</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+plasma%22">Blood plasma</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: A reversed phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors (NRTIs) lamivudine, didanosine, stavudine, zidovudine and abacavir in plasma. The method involved solid-phase extraction with Oasis MAX cartridges from plasma, followed by high performance liquid chromatography with a SymmetryShield RP 18 column and ultraviolet detection set at a wavelength of 260nm. The assay was validated over the concentration range of 0.015–5mg/l for all five NRTIs. The average accuracies for the assay were 92–102%, inter- and intra-day coefficients of variation (CV) were <2.5% and extraction recoveries were higher than 97%. This method proved to be simple, accurate and precise, and is currently in use in our laboratory for the quantitative analysis of NRTIs in plasma. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=16134182 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jchromb.2004.11.019 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 121 Subjects: – SubjectFull: High performance liquid chromatography Type: general – SubjectFull: Solid-phase analysis Type: general – SubjectFull: Ultraviolet radiation Type: general – SubjectFull: Nucleosides Type: general – SubjectFull: Blood plasma Type: general Titles: – TitleFull: Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Verweij-van Wissen, C.P.W.G.M. – PersonEntity: Name: NameFull: Aarnoutse, R.E. – PersonEntity: Name: NameFull: Burger, D.M. IsPartOfRelationships: – BibEntity: Dates: – D: 25 M: 02 Text: Feb2005 Type: published Y: 2005 Identifiers: – Type: issn-print Value: 15700232 Numbering: – Type: volume Value: 816 – Type: issue Value: 1/2 Titles: – TitleFull: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences Type: main |
| ResultId | 1 |
