View in EDS

Rapid adaptive evolution of Bacillus coagulans to undetoxified corncob hydrolysates for lactic acid production and new insights into its high phenolic degradation.

Saved in:
Bibliographic Details
Title: Rapid adaptive evolution of Bacillus coagulans to undetoxified corncob hydrolysates for lactic acid production and new insights into its high phenolic degradation.
Authors: Fu, Jiaming1 (AUTHOR), Wang, Zijie1 (AUTHOR), Miao, Hongcheng1 (AUTHOR), Yu, Chang1 (AUTHOR), Zheng, Zhaojuan1 (AUTHOR), Ouyang, Jia1 (AUTHOR) hgouyj@njfu.edu.cn
Source: Bioresource Technology. Sep2023, Vol. 383, pN.PAG-N.PAG. 1p.
Subjects: Biological evolution, Bacillus (Bacteria), Lactic acid, Corncobs, Phenolic acids, Lignocellulose, Ferulic acid, Phenols
Abstract: [Display omitted] • Bacillus coagulans CC17B-1 can produce LA directly from corn cob hydrolysates. • CC17B-1 demonstrated a remarkable self-detoxification ability for hydrolysates. • Highly efficient phenolic acids degradation capacity was observed. • Phenolic aldehydes are converted to phenolic alcohols, which are further degraded. Here, an adapted Bacillus coagulans (Weizmannia coagulans) strain CC17B-1 was developed for lignocellulosic lactic acid production through a short and rapid adaptive laboratory evolution technique. Without any detoxification, two actual corn cob hydrolysates from the factory were effectively fermented to lactic acid within 60 h. Strain CC17B-1 is capable of degrading all nine determined phenolic compounds in the hydrolysate, with the only exception being vanillic acid. Notably, its tolerances for ferulic acid and p -coumaric acid are the highest doses reported in anaerobic microbes. A proposed degradation pathway showed that strain CC17B-1 could convert phenolic aldehydes to phenolic alcohol and then further degrade them completely. This work provides new ideas for the microbe phenolic degradation pathway and paves the way for industrial lactic acid production from lignocellulosic biomass. [ABSTRACT FROM AUTHOR]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:[Display omitted] • Bacillus coagulans CC17B-1 can produce LA directly from corn cob hydrolysates. • CC17B-1 demonstrated a remarkable self-detoxification ability for hydrolysates. • Highly efficient phenolic acids degradation capacity was observed. • Phenolic aldehydes are converted to phenolic alcohols, which are further degraded. Here, an adapted Bacillus coagulans (Weizmannia coagulans) strain CC17B-1 was developed for lignocellulosic lactic acid production through a short and rapid adaptive laboratory evolution technique. Without any detoxification, two actual corn cob hydrolysates from the factory were effectively fermented to lactic acid within 60 h. Strain CC17B-1 is capable of degrading all nine determined phenolic compounds in the hydrolysate, with the only exception being vanillic acid. Notably, its tolerances for ferulic acid and p -coumaric acid are the highest doses reported in anaerobic microbes. A proposed degradation pathway showed that strain CC17B-1 could convert phenolic aldehydes to phenolic alcohol and then further degrade them completely. This work provides new ideas for the microbe phenolic degradation pathway and paves the way for industrial lactic acid production from lignocellulosic biomass. [ABSTRACT FROM AUTHOR]
ISSN:09608524
DOI:10.1016/j.biortech.2023.129246