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Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages.

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Title: Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages.
Authors: Young, Adam1,2, Neumann, Bjoern1,2, Segel, Michael1, Zi-Yu Chen, Civia1,2, Tourlomousis, Panagiotis3, Franklin, Robin J. M.1,2 rfranklin@altoslabs.com
Source: Proceedings of the National Academy of Sciences of the United States of America. 8/29/2023, Vol. 120 Issue 35, p1-3. 6p.
Subjects: Adeno-associated virus, Microglia, Macrophages, Capsids, Transgene expression
Abstract: Tissue macrophages, including microglia, are notoriously resistant to genetic manipulation. Here, we report the creation of Adeno-associated viruses (AAV) variants that efficiently and widely transduce microglia and tissue macrophages in vivo following intravenous delivery, with transgene expression of up to 80%. We use this technology to demonstrate manipulation of microglia gene expression and microglial ablation, thereby providing invaluable research tools for the study of these important cells. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 171798172
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages.
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  Data: <searchLink fieldCode="DE" term="%22Adeno-associated+virus%22">Adeno-associated virus</searchLink><br /><searchLink fieldCode="DE" term="%22Microglia%22">Microglia</searchLink><br /><searchLink fieldCode="DE" term="%22Macrophages%22">Macrophages</searchLink><br /><searchLink fieldCode="DE" term="%22Capsids%22">Capsids</searchLink><br /><searchLink fieldCode="DE" term="%22Transgene+expression%22">Transgene expression</searchLink>
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  Data: Tissue macrophages, including microglia, are notoriously resistant to genetic manipulation. Here, we report the creation of Adeno-associated viruses (AAV) variants that efficiently and widely transduce microglia and tissue macrophages in vivo following intravenous delivery, with transgene expression of up to 80%. We use this technology to demonstrate manipulation of microglia gene expression and microglial ablation, thereby providing invaluable research tools for the study of these important cells. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=171798172
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        Value: 10.1073/pnas.2302997120
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        Text: English
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        Type: general
      – SubjectFull: Microglia
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      – SubjectFull: Macrophages
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      – SubjectFull: Capsids
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      – SubjectFull: Transgene expression
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              Text: 8/29/2023
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