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Isolation and characterisation of an environmental Clostridium beijerinckii strain for biohydrogen production from dairy wastes.

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Title: Isolation and characterisation of an environmental Clostridium beijerinckii strain for biohydrogen production from dairy wastes.
Authors: Mete, M.1,2,3 (AUTHOR) mayumi.mete@imt-atlantique.fr, Pattyn, P.2 (AUTHOR), Robidart, A.2 (AUTHOR), Beringuier, G.2 (AUTHOR), Thomas, H.2 (AUTHOR), Grandjean, C.3 (AUTHOR), Irague, R.2 (AUTHOR), Andres, Y.1 (AUTHOR)
Source: International Journal of Hydrogen Energy. Jan2024:Part A, Vol. 49, p371-383. 13p.
Subjects: Dairy waste, Industrial wastes, Cattle manure, Clostridium, Dairy farms, Maltose, Butanol, Sucrose, Hydrogen as fuel
Abstract: Biological dihydrogen (H 2) production is a promising alternative to the conventional non-renewable energies. H 2 production by dark fermentation is particularly studied regarding its independence from fossil fuel and electricity, its cultivation into bioreactors and the broad range of substrate that can be used. A major goal application of dark fermentation is the valuation of wastes. In this context, cheese whey wastes are particularly adapted, since rich in organic matter, abundant and low cost. A novel H 2 -producing strain was isolated from a sample of mixed bovine manure and cheese whey, collected in a cheese-producing dairy farm. The strain was identified as a Clostridium beijerinckii strain based on morphological and physiological characteristics, and 16S rDNA sequencing. The optimum temperature and pH for H 2 production was 40 °C and pH 7, respectively. Substrate and stress tolerance tests showed that C. beijerinckii C.sp.1.3 could produce H 2 from glucose, lactose, maltose, cellobiose, galacturonic acid, xylose, and sucrose, and to a lesser extent from starch, cellulose and glycerol. The H 2 production potential from a mix of dairy industrial wastes of this strain has been evaluated. The highest productivity in batch for 5 g L−1 of Total Organic Carbon (TOC) was 833.1 mL H 2 L−1 d−1, with a yield of 2.03 mM H2 mM lactose −1, demonstrating the potential of this strain for H 2 production from industrial wastes. • An H 2 -producing strain was isolated from a mixture of manure and dairy waste. • The strain has the potential of fermenting a broad range of economic substrates. • Mixture of crude cheese whey and wastewater were used as model for H 2 production. • Productivity reach up to 833.1 mL L−1 d−1 with a yield of 2.03 mol H2.mol lactose −1. • A mechanism of lactate-driven fermentation was observed. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Biological dihydrogen (H 2) production is a promising alternative to the conventional non-renewable energies. H 2 production by dark fermentation is particularly studied regarding its independence from fossil fuel and electricity, its cultivation into bioreactors and the broad range of substrate that can be used. A major goal application of dark fermentation is the valuation of wastes. In this context, cheese whey wastes are particularly adapted, since rich in organic matter, abundant and low cost. A novel H 2 -producing strain was isolated from a sample of mixed bovine manure and cheese whey, collected in a cheese-producing dairy farm. The strain was identified as a Clostridium beijerinckii strain based on morphological and physiological characteristics, and 16S rDNA sequencing. The optimum temperature and pH for H 2 production was 40 °C and pH 7, respectively. Substrate and stress tolerance tests showed that C. beijerinckii C.sp.1.3 could produce H 2 from glucose, lactose, maltose, cellobiose, galacturonic acid, xylose, and sucrose, and to a lesser extent from starch, cellulose and glycerol. The H 2 production potential from a mix of dairy industrial wastes of this strain has been evaluated. The highest productivity in batch for 5 g L−1 of Total Organic Carbon (TOC) was 833.1 mL H 2 L−1 d−1, with a yield of 2.03 mM H2 mM lactose −1, demonstrating the potential of this strain for H 2 production from industrial wastes. • An H 2 -producing strain was isolated from a mixture of manure and dairy waste. • The strain has the potential of fermenting a broad range of economic substrates. • Mixture of crude cheese whey and wastewater were used as model for H 2 production. • Productivity reach up to 833.1 mL L−1 d−1 with a yield of 2.03 mol H2.mol lactose −1. • A mechanism of lactate-driven fermentation was observed. [ABSTRACT FROM AUTHOR]
ISSN:03603199
DOI:10.1016/j.ijhydene.2023.08.274