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Rapid and label-free A2 peptide epitope decorated CoFe2O4-C60 nanocomposite-based electrochemical immunosensor for detecting Visceral Leishmaniasis.

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Title: Rapid and label-free A2 peptide epitope decorated CoFe2O4-C60 nanocomposite-based electrochemical immunosensor for detecting Visceral Leishmaniasis.
Authors: Adu, Darko Kwabena1 (AUTHOR), Nate, Zondi2 (AUTHOR), Alake, John1 (AUTHOR), Ike, Blessing Wisdom1 (AUTHOR), Mahlalela, Mavela Cleopus1 (AUTHOR), Mohite, Sachin Balaso1 (AUTHOR), Mokoena, Sithabile1 (AUTHOR), Chauhan, Ruchika1 (AUTHOR), Karpoormath, Rajshekhar1 (AUTHOR) karpoormath@ukzn.ac.za
Source: Bioelectrochemistry. Jun2024, Vol. 157, pN.PAG-N.PAG. 1p.
Subjects: Great Britain. National Health Service, Visceral leishmaniasis, Peptides, Energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, High performance liquid chromatography
Abstract: • Flame synthesis and characterisation of CoFe 2 O 4 -C60NP. • Synthesis and characterisation of A2 peptide antigen using LCMS and HPLC. • A2 peptide antigen decorated CoFe 2 O 4 -C60NP immunoelectrode. • Immunosensor with 30.34 fg/mL LOD at a linear range of 10−10 to 10−1 µg/mL. • Label-free electrochemical detection of A2-antibodies in spiked blood serum. Diagnosis of Visceral Leishmaniasis is challenging due to the shared clinical features with malaria, typhoid, and tuberculosis. A CoFe 2 O 4 -C60 nanocomposite-based immunosensor decorated with a sensitive A2 peptide antigen was fabricated to detect anti-A2 antibodies for application in visceral leishmaniasis diagnosis. The flame-synthesised nanocomposite was characterised using Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) techniques. N terminated specific A2 peptide epitope antigen (NH 2 -QSVGPLSVGP-OH) was synthesised and characterised by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS). Using EDC/NHS, A2 peptide antigen (Apg) was immobilised on the CoFe 2 O 4 -C60-modified electrode. The performance of the immunosensor, Apg-CoFe 2 O 4 -C60NP/GCE, was evaluated by testing its ability to detect varying concentrations of anti-A2 antibody solution in PBS and spiked serum with 1 mM [Fe(CN) 6 ]3−/4− in 0.01 M PBS (pH 7.4) as supporting electrolyte. using differential pulse voltammetry. The immunosensor showed excellent reproducibility and a linear range of 10−10–10−1 µg/mL, with an experimental detection limit of 30.34 fg/mL. These results suggest that the fabricated sensor has great potential as a tool for diagnosing visceral leishmaniasis. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:• Flame synthesis and characterisation of CoFe 2 O 4 -C60NP. • Synthesis and characterisation of A2 peptide antigen using LCMS and HPLC. • A2 peptide antigen decorated CoFe 2 O 4 -C60NP immunoelectrode. • Immunosensor with 30.34 fg/mL LOD at a linear range of 10−10 to 10−1 µg/mL. • Label-free electrochemical detection of A2-antibodies in spiked blood serum. Diagnosis of Visceral Leishmaniasis is challenging due to the shared clinical features with malaria, typhoid, and tuberculosis. A CoFe 2 O 4 -C60 nanocomposite-based immunosensor decorated with a sensitive A2 peptide antigen was fabricated to detect anti-A2 antibodies for application in visceral leishmaniasis diagnosis. The flame-synthesised nanocomposite was characterised using Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) techniques. N terminated specific A2 peptide epitope antigen (NH 2 -QSVGPLSVGP-OH) was synthesised and characterised by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS). Using EDC/NHS, A2 peptide antigen (Apg) was immobilised on the CoFe 2 O 4 -C60-modified electrode. The performance of the immunosensor, Apg-CoFe 2 O 4 -C60NP/GCE, was evaluated by testing its ability to detect varying concentrations of anti-A2 antibody solution in PBS and spiked serum with 1 mM [Fe(CN) 6 ]3−/4− in 0.01 M PBS (pH 7.4) as supporting electrolyte. using differential pulse voltammetry. The immunosensor showed excellent reproducibility and a linear range of 10−10–10−1 µg/mL, with an experimental detection limit of 30.34 fg/mL. These results suggest that the fabricated sensor has great potential as a tool for diagnosing visceral leishmaniasis. [ABSTRACT FROM AUTHOR]
ISSN:15675394
DOI:10.1016/j.bioelechem.2024.108662