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Rapid and label-free A2 peptide epitope decorated CoFe2O4-C60 nanocomposite-based electrochemical immunosensor for detecting Visceral Leishmaniasis.

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Title: Rapid and label-free A2 peptide epitope decorated CoFe2O4-C60 nanocomposite-based electrochemical immunosensor for detecting Visceral Leishmaniasis.
Authors: Adu, Darko Kwabena1 (AUTHOR), Nate, Zondi2 (AUTHOR), Alake, John1 (AUTHOR), Ike, Blessing Wisdom1 (AUTHOR), Mahlalela, Mavela Cleopus1 (AUTHOR), Mohite, Sachin Balaso1 (AUTHOR), Mokoena, Sithabile1 (AUTHOR), Chauhan, Ruchika1 (AUTHOR), Karpoormath, Rajshekhar1 (AUTHOR) karpoormath@ukzn.ac.za
Source: Bioelectrochemistry. Jun2024, Vol. 157, pN.PAG-N.PAG. 1p.
Subjects: Great Britain. National Health Service, Visceral leishmaniasis, Peptides, Energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, High performance liquid chromatography
Abstract: • Flame synthesis and characterisation of CoFe 2 O 4 -C60NP. • Synthesis and characterisation of A2 peptide antigen using LCMS and HPLC. • A2 peptide antigen decorated CoFe 2 O 4 -C60NP immunoelectrode. • Immunosensor with 30.34 fg/mL LOD at a linear range of 10−10 to 10−1 µg/mL. • Label-free electrochemical detection of A2-antibodies in spiked blood serum. Diagnosis of Visceral Leishmaniasis is challenging due to the shared clinical features with malaria, typhoid, and tuberculosis. A CoFe 2 O 4 -C60 nanocomposite-based immunosensor decorated with a sensitive A2 peptide antigen was fabricated to detect anti-A2 antibodies for application in visceral leishmaniasis diagnosis. The flame-synthesised nanocomposite was characterised using Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) techniques. N terminated specific A2 peptide epitope antigen (NH 2 -QSVGPLSVGP-OH) was synthesised and characterised by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS). Using EDC/NHS, A2 peptide antigen (Apg) was immobilised on the CoFe 2 O 4 -C60-modified electrode. The performance of the immunosensor, Apg-CoFe 2 O 4 -C60NP/GCE, was evaluated by testing its ability to detect varying concentrations of anti-A2 antibody solution in PBS and spiked serum with 1 mM [Fe(CN) 6 ]3−/4− in 0.01 M PBS (pH 7.4) as supporting electrolyte. using differential pulse voltammetry. The immunosensor showed excellent reproducibility and a linear range of 10−10–10−1 µg/mL, with an experimental detection limit of 30.34 fg/mL. These results suggest that the fabricated sensor has great potential as a tool for diagnosing visceral leishmaniasis. [ABSTRACT FROM AUTHOR]
Copyright of Bioelectrochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Rapid and label-free A2 peptide epitope decorated CoFe2O4-C60 nanocomposite-based electrochemical immunosensor for detecting Visceral Leishmaniasis.
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  Data: <searchLink fieldCode="AR" term="%22Adu%2C+Darko+Kwabena%22">Adu, Darko Kwabena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nate%2C+Zondi%22">Nate, Zondi</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alake%2C+John%22">Alake, John</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ike%2C+Blessing+Wisdom%22">Ike, Blessing Wisdom</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mahlalela%2C+Mavela+Cleopus%22">Mahlalela, Mavela Cleopus</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mohite%2C+Sachin+Balaso%22">Mohite, Sachin Balaso</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mokoena%2C+Sithabile%22">Mokoena, Sithabile</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chauhan%2C+Ruchika%22">Chauhan, Ruchika</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Karpoormath%2C+Rajshekhar%22">Karpoormath, Rajshekhar</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> karpoormath@ukzn.ac.za</i>
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  Data: <searchLink fieldCode="DE" term="%22Great+Britain%2E+National+Health+Service%22">Great Britain. National Health Service</searchLink><br /><searchLink fieldCode="DE" term="%22Visceral+leishmaniasis%22">Visceral leishmaniasis</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Energy+dispersive+X-ray+spectroscopy%22">Energy dispersive X-ray spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Fourier+transform+infrared+spectroscopy%22">Fourier transform infrared spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink>
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  Label: Abstract
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  Data: • Flame synthesis and characterisation of CoFe 2 O 4 -C60NP. • Synthesis and characterisation of A2 peptide antigen using LCMS and HPLC. • A2 peptide antigen decorated CoFe 2 O 4 -C60NP immunoelectrode. • Immunosensor with 30.34 fg/mL LOD at a linear range of 10−10 to 10−1 µg/mL. • Label-free electrochemical detection of A2-antibodies in spiked blood serum. Diagnosis of Visceral Leishmaniasis is challenging due to the shared clinical features with malaria, typhoid, and tuberculosis. A CoFe 2 O 4 -C60 nanocomposite-based immunosensor decorated with a sensitive A2 peptide antigen was fabricated to detect anti-A2 antibodies for application in visceral leishmaniasis diagnosis. The flame-synthesised nanocomposite was characterised using Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) techniques. N terminated specific A2 peptide epitope antigen (NH 2 -QSVGPLSVGP-OH) was synthesised and characterised by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS). Using EDC/NHS, A2 peptide antigen (Apg) was immobilised on the CoFe 2 O 4 -C60-modified electrode. The performance of the immunosensor, Apg-CoFe 2 O 4 -C60NP/GCE, was evaluated by testing its ability to detect varying concentrations of anti-A2 antibody solution in PBS and spiked serum with 1 mM [Fe(CN) 6 ]3−/4− in 0.01 M PBS (pH 7.4) as supporting electrolyte. using differential pulse voltammetry. The immunosensor showed excellent reproducibility and a linear range of 10−10–10−1 µg/mL, with an experimental detection limit of 30.34 fg/mL. These results suggest that the fabricated sensor has great potential as a tool for diagnosing visceral leishmaniasis. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Bioelectrochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.bioelechem.2024.108662
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Great Britain. National Health Service
        Type: general
      – SubjectFull: Visceral leishmaniasis
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      – SubjectFull: Peptides
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      – SubjectFull: Energy dispersive X-ray spectroscopy
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      – SubjectFull: Fourier transform infrared spectroscopy
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      – SubjectFull: High performance liquid chromatography
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      – TitleFull: Rapid and label-free A2 peptide epitope decorated CoFe2O4-C60 nanocomposite-based electrochemical immunosensor for detecting Visceral Leishmaniasis.
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              Text: Jun2024
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