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In vivo self-assembly of an intact functional cage protein: Intracellular generation of chimeric ferritins without disassembly-involved damage.

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Title: In vivo self-assembly of an intact functional cage protein: Intracellular generation of chimeric ferritins without disassembly-involved damage.
Authors: Kim, Koung Hee1 (AUTHOR), Ki, Mi-Ran1,2 (AUTHOR), Nguyen, Thi Khoa My1 (AUTHOR), Min, Ki Ha1,2 (AUTHOR), Pack, Seung Pil1 (AUTHOR) spack@korea.ac.kr
Source: Journal of Industrial & Engineering Chemistry. Jan2025, Vol. 141, p67-71. 5p.
Subjects: Peptides, Functional analysis, Monomers, Design templates, Nanostructured materials, Ferritin
Abstract: [Display omitted] Ferritin (Fn) is an attractive nano-biomaterial with a hollow structure. Functional chimeric cage-protein Fns (chimera-Fn) can be designed by combining two different monomer subunits. However, unavoidable damage is induced by the severe disassembly conditions required for preparing the Fn monomer. Here, a biocompatible method using a designed dual-expression vector to obtain chimera-Fns without a damage-inducing process is described. Fn monomer and silica-forming peptide (Kpt)-fused Fn monomer are expressed simultaneously and assembled within host-cells to form chimera-Fn of Kpt. Characteristic analysis showed that the chimera-Fns obtained intracellularly were composed of the two monomers in a ratio of 1:1. Morphological and functional analyses determined that the intracellularly-generated chimera-Fns had more intact forms and bioactive functions compared with those produced by chemical-involved disassembly and reassembly. This is the first report about the in vivo self-assembly of chimera-Fn with intact function and hold promise in designing functional templates for various nanomaterial preparations. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:[Display omitted] Ferritin (Fn) is an attractive nano-biomaterial with a hollow structure. Functional chimeric cage-protein Fns (chimera-Fn) can be designed by combining two different monomer subunits. However, unavoidable damage is induced by the severe disassembly conditions required for preparing the Fn monomer. Here, a biocompatible method using a designed dual-expression vector to obtain chimera-Fns without a damage-inducing process is described. Fn monomer and silica-forming peptide (Kpt)-fused Fn monomer are expressed simultaneously and assembled within host-cells to form chimera-Fn of Kpt. Characteristic analysis showed that the chimera-Fns obtained intracellularly were composed of the two monomers in a ratio of 1:1. Morphological and functional analyses determined that the intracellularly-generated chimera-Fns had more intact forms and bioactive functions compared with those produced by chemical-involved disassembly and reassembly. This is the first report about the in vivo self-assembly of chimera-Fn with intact function and hold promise in designing functional templates for various nanomaterial preparations. [ABSTRACT FROM AUTHOR]
ISSN:1226086X
DOI:10.1016/j.jiec.2024.06.040