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Design and biofabrication of barnacle and spider silk protein decorated composite bacterial cellulose for diabetic wound healing.

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Bibliographic Details
Title: Design and biofabrication of barnacle and spider silk protein decorated composite bacterial cellulose for diabetic wound healing.
Authors: Ye, Luona1 (AUTHOR), Yan, Yunjun1 (AUTHOR) yanyunjun@hust.edu.cn, Yan, Jinyong1 (AUTHOR) yjiny@126.com
Source: Carbohydrate Polymers. Apr2025, Vol. 354, pN.PAG-N.PAG. 1p.
Subjects: Laboratory rats, Spider silk, Wound healing, Hyaluronic acid, Hydrogels, Bacterial diseases
Abstract: Delayed healing of wounds in diabetics is mainly due to tissue inflammation, poor vasculature, lack of neovascularization, and bacterial infection. Therefore, a therapeutic protocol that disrupts this cycle and speeds healing is urgently needed. Despite attempts to enhance wound dressing effectiveness through hydrogels with diverse complexes such as bacterial cellulose (BC) combined with chitosan, BC/ chitosan/hyaluronic acid, and BC/chitosan/collagen, the toughness and adhesion properties of hydrogel remain constrained, leading to inadequate and uncontrollable wound healing. To address the challenge, we have devised an innovative solution by integrating barnacle cement protein (cp19k) and spider silk protein (major ampullate spidroin 1, MaSp1) into a BC matrix, complemented by chitosan. This development has led to the creation of a novel BC-based composite hydrogel BC/cp19k-MaSp1/C 150k. The composite hydrogel stands out with its remarkable mechanical (3.92 Mpa) and adhesion properties (8.4 kPa) compared to its BC/C 150k counterpart. Meanwhile, the BC/cp19k-MaSp1/C 150k hydrogel also demonstrated antimicrobial activity, coagulation, and biocompatibility. The BC/cp19k-MaSp1/C 150k hydrogel showed an exceptional capacity to enhance wound healing in a diabetic rat model, achieving a significant wound closure rate of over 98 % on day 14 when compared to BC and commercially available dressing 3 M™ Tegaderm™. This advancement holds significant promise in revolutionizing wound management for diabetics. This study synthesized a BC-based nanocomplex hydrogel for diabetic wound healing. BC/cp19k-MaSp1/C 150k hydrogel showed strong mechanical and adhesion properties, antimicrobial activity, and enhanced wound healing in diabetic rats, indicating its potential as a biomedical dressing. [Display omitted] [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Delayed healing of wounds in diabetics is mainly due to tissue inflammation, poor vasculature, lack of neovascularization, and bacterial infection. Therefore, a therapeutic protocol that disrupts this cycle and speeds healing is urgently needed. Despite attempts to enhance wound dressing effectiveness through hydrogels with diverse complexes such as bacterial cellulose (BC) combined with chitosan, BC/ chitosan/hyaluronic acid, and BC/chitosan/collagen, the toughness and adhesion properties of hydrogel remain constrained, leading to inadequate and uncontrollable wound healing. To address the challenge, we have devised an innovative solution by integrating barnacle cement protein (cp19k) and spider silk protein (major ampullate spidroin 1, MaSp1) into a BC matrix, complemented by chitosan. This development has led to the creation of a novel BC-based composite hydrogel BC/cp19k-MaSp1/C 150k. The composite hydrogel stands out with its remarkable mechanical (3.92 Mpa) and adhesion properties (8.4 kPa) compared to its BC/C 150k counterpart. Meanwhile, the BC/cp19k-MaSp1/C 150k hydrogel also demonstrated antimicrobial activity, coagulation, and biocompatibility. The BC/cp19k-MaSp1/C 150k hydrogel showed an exceptional capacity to enhance wound healing in a diabetic rat model, achieving a significant wound closure rate of over 98 % on day 14 when compared to BC and commercially available dressing 3 M™ Tegaderm™. This advancement holds significant promise in revolutionizing wound management for diabetics. This study synthesized a BC-based nanocomplex hydrogel for diabetic wound healing. BC/cp19k-MaSp1/C 150k hydrogel showed strong mechanical and adhesion properties, antimicrobial activity, and enhanced wound healing in diabetic rats, indicating its potential as a biomedical dressing. [Display omitted] [ABSTRACT FROM AUTHOR]
ISSN:01448617
DOI:10.1016/j.carbpol.2025.123301