Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite.
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| Title: | Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite. |
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| Authors: | Chaudhary, Shikha1,2 (AUTHOR), Maurya, Anjali1 (AUTHOR), Das, Uddipan3 (AUTHOR), Tripathi, Ravi Mani2 (AUTHOR), Yadav, Subhash Chandra1 (AUTHOR) subhashmbu@aiims.gov.in |
| Source: | Journal of Nanoparticle Research. Feb2025, Vol. 27 Issue 2, p1-16. 16p. |
| Subjects: | Circular dichroism, Particle analysis, Quantum dots, Thermal stability, Molecular interactions |
| Abstract: | The thermal stability of core–shell quantum dots (QDs) encapsulated apoferritin has been sparingly reported. In this study, we reported the spontaneous encapsulation of mercaptopropionic acid functionalized CdSe:CdS:ZnS core–shell QDs and temperature-induced structural changes of QDs-apoferritin composite using biophysical techniques and negative stain single particle analysis. An increase in absorbance and decrease in tryptophan fluorescence intensity was observed by increasing QDs concentration (0–250 ng/mL). A change in circular dichroism characteristic peaks was observed with increasing temperatures (25 °C, 37 °C, and 55 °C). HR-TEM image also showed an increase in size (12.0 ± 1.0 nm at 25 °C, 12.5 ± 1.0 nm at 37 °C, and 15 ± 1.3 nm at 55 °C) along with 6 ± 1% and 68 ± 5% release of QDs (than 25 °C) from the composite at 37 °C and 55 °C, respectively. The single particle analysis confirmed the encapsulation of four QD particles at 25 °C but showed multiple 2D class averages at 37 °C, confirming the destabilization and molten globule-like structure at 55 °C. This study revealed that QDs induced significant structural alteration in the apoferritin at a much lower temperature than its normal melting temperature (80 °C). [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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| Abstract: | The thermal stability of core–shell quantum dots (QDs) encapsulated apoferritin has been sparingly reported. In this study, we reported the spontaneous encapsulation of mercaptopropionic acid functionalized CdSe:CdS:ZnS core–shell QDs and temperature-induced structural changes of QDs-apoferritin composite using biophysical techniques and negative stain single particle analysis. An increase in absorbance and decrease in tryptophan fluorescence intensity was observed by increasing QDs concentration (0–250 ng/mL). A change in circular dichroism characteristic peaks was observed with increasing temperatures (25 °C, 37 °C, and 55 °C). HR-TEM image also showed an increase in size (12.0 ± 1.0 nm at 25 °C, 12.5 ± 1.0 nm at 37 °C, and 15 ± 1.3 nm at 55 °C) along with 6 ± 1% and 68 ± 5% release of QDs (than 25 °C) from the composite at 37 °C and 55 °C, respectively. The single particle analysis confirmed the encapsulation of four QD particles at 25 °C but showed multiple 2D class averages at 37 °C, confirming the destabilization and molten globule-like structure at 55 °C. This study revealed that QDs induced significant structural alteration in the apoferritin at a much lower temperature than its normal melting temperature (80 °C). [ABSTRACT FROM AUTHOR] |
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| ISSN: | 13880764 |
| DOI: | 10.1007/s11051-025-06218-0 |
