Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite.
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| Title: | Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite. |
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| Authors: | Chaudhary, Shikha1,2 (AUTHOR), Maurya, Anjali1 (AUTHOR), Das, Uddipan3 (AUTHOR), Tripathi, Ravi Mani2 (AUTHOR), Yadav, Subhash Chandra1 (AUTHOR) subhashmbu@aiims.gov.in |
| Source: | Journal of Nanoparticle Research. Feb2025, Vol. 27 Issue 2, p1-16. 16p. |
| Subjects: | Circular dichroism, Particle analysis, Quantum dots, Thermal stability, Molecular interactions |
| Abstract: | The thermal stability of core–shell quantum dots (QDs) encapsulated apoferritin has been sparingly reported. In this study, we reported the spontaneous encapsulation of mercaptopropionic acid functionalized CdSe:CdS:ZnS core–shell QDs and temperature-induced structural changes of QDs-apoferritin composite using biophysical techniques and negative stain single particle analysis. An increase in absorbance and decrease in tryptophan fluorescence intensity was observed by increasing QDs concentration (0–250 ng/mL). A change in circular dichroism characteristic peaks was observed with increasing temperatures (25 °C, 37 °C, and 55 °C). HR-TEM image also showed an increase in size (12.0 ± 1.0 nm at 25 °C, 12.5 ± 1.0 nm at 37 °C, and 15 ± 1.3 nm at 55 °C) along with 6 ± 1% and 68 ± 5% release of QDs (than 25 °C) from the composite at 37 °C and 55 °C, respectively. The single particle analysis confirmed the encapsulation of four QD particles at 25 °C but showed multiple 2D class averages at 37 °C, confirming the destabilization and molten globule-like structure at 55 °C. This study revealed that QDs induced significant structural alteration in the apoferritin at a much lower temperature than its normal melting temperature (80 °C). [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Nanoparticle Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 183405814 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chaudhary%2C+Shikha%22">Chaudhary, Shikha</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maurya%2C+Anjali%22">Maurya, Anjali</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Das%2C+Uddipan%22">Das, Uddipan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tripathi%2C+Ravi+Mani%22">Tripathi, Ravi Mani</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yadav%2C+Subhash+Chandra%22">Yadav, Subhash Chandra</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> subhashmbu@aiims.gov.in</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Nanoparticle+Research%22">Journal of Nanoparticle Research</searchLink>. Feb2025, Vol. 27 Issue 2, p1-16. 16p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Circular+dichroism%22">Circular dichroism</searchLink><br /><searchLink fieldCode="DE" term="%22Particle+analysis%22">Particle analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Quantum+dots%22">Quantum dots</searchLink><br /><searchLink fieldCode="DE" term="%22Thermal+stability%22">Thermal stability</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+interactions%22">Molecular interactions</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The thermal stability of core–shell quantum dots (QDs) encapsulated apoferritin has been sparingly reported. In this study, we reported the spontaneous encapsulation of mercaptopropionic acid functionalized CdSe:CdS:ZnS core–shell QDs and temperature-induced structural changes of QDs-apoferritin composite using biophysical techniques and negative stain single particle analysis. An increase in absorbance and decrease in tryptophan fluorescence intensity was observed by increasing QDs concentration (0–250 ng/mL). A change in circular dichroism characteristic peaks was observed with increasing temperatures (25 °C, 37 °C, and 55 °C). HR-TEM image also showed an increase in size (12.0 ± 1.0 nm at 25 °C, 12.5 ± 1.0 nm at 37 °C, and 15 ± 1.3 nm at 55 °C) along with 6 ± 1% and 68 ± 5% release of QDs (than 25 °C) from the composite at 37 °C and 55 °C, respectively. The single particle analysis confirmed the encapsulation of four QD particles at 25 °C but showed multiple 2D class averages at 37 °C, confirming the destabilization and molten globule-like structure at 55 °C. This study revealed that QDs induced significant structural alteration in the apoferritin at a much lower temperature than its normal melting temperature (80 °C). [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Nanoparticle Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s11051-025-06218-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 16 StartPage: 1 Subjects: – SubjectFull: Circular dichroism Type: general – SubjectFull: Particle analysis Type: general – SubjectFull: Quantum dots Type: general – SubjectFull: Thermal stability Type: general – SubjectFull: Molecular interactions Type: general Titles: – TitleFull: Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chaudhary, Shikha – PersonEntity: Name: NameFull: Maurya, Anjali – PersonEntity: Name: NameFull: Das, Uddipan – PersonEntity: Name: NameFull: Tripathi, Ravi Mani – PersonEntity: Name: NameFull: Yadav, Subhash Chandra IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 13880764 Numbering: – Type: volume Value: 27 – Type: issue Value: 2 Titles: – TitleFull: Journal of Nanoparticle Research Type: main |
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