Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model.
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| Title: | Hybrid Implants Based on Calcium–Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and rS1/9 Recombinant Spidroin as a Scaffold: Reparative Osteogenesis in a Mouse Craniotomy Model. |
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| Authors: | Karyagina, A. S.1,2,3 (AUTHOR), Bogush, V. G.3,4 (AUTHOR) vlbogush@mail.ru, Orlova, P. A.1 (AUTHOR), Davydova, L. I.4 (AUTHOR), Zhulina, A. V.1 (AUTHOR), Grunina, T. M.1,3 (AUTHOR), Strukova, N. V.1 (AUTHOR), Generalova, M. S.1 (AUTHOR), Krivozubov, M. S.1 (AUTHOR), Cheperegin, S. E.4 (AUTHOR), Ramonova, A. A.5 (AUTHOR), Gromov, A. V.1 (AUTHOR) |
| Source: | Applied Biochemistry & Microbiology. Dec2024, Vol. 60 Issue 7, p1493-1503. 11p. |
| Subjects: | Oxide ceramics, Diopside, Hyaluronic acid, Bone growth, Bone marrow |
| Abstract: | Abstract—Calcium–magnesium silicate ceramic diopside is an effective carrier of BMP-2 and, in combination with a bovine demineralized bone matrix as a scaffold, can induce effective reparative osteogenesis on the model of craniotomy in mice. It was of interest to investigate the efficacy of combined usage of BMP-2-loaded diopside with recombinant spidroins for the induction of osteogenesis. For this purpose, porous implants were prepared based on recombinant spidroin rS1/9, into which BMP-2 was introduced either as BMP-2 loaded diopside particles suspended in hyaluronic acid or by adsorption. Neo-osteogenesis with bone marrow formation occurred in both groups with BMP-2, most pronounced in the group with BMP-2 and diopside on the model of critical size defects in the cranium in mice. Thus, the use of 3D scaffolds based on recombinant spidroin with BMP2-loaded diopside particles in hyaluronic acid can lead to effective induction of osteogenesis. The spidroin scaffolds themselves represent a sufficiently effective carrier for BMP-2. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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| Abstract: | Abstract—Calcium–magnesium silicate ceramic diopside is an effective carrier of BMP-2 and, in combination with a bovine demineralized bone matrix as a scaffold, can induce effective reparative osteogenesis on the model of craniotomy in mice. It was of interest to investigate the efficacy of combined usage of BMP-2-loaded diopside with recombinant spidroins for the induction of osteogenesis. For this purpose, porous implants were prepared based on recombinant spidroin rS1/9, into which BMP-2 was introduced either as BMP-2 loaded diopside particles suspended in hyaluronic acid or by adsorption. Neo-osteogenesis with bone marrow formation occurred in both groups with BMP-2, most pronounced in the group with BMP-2 and diopside on the model of critical size defects in the cranium in mice. Thus, the use of 3D scaffolds based on recombinant spidroin with BMP2-loaded diopside particles in hyaluronic acid can lead to effective induction of osteogenesis. The spidroin scaffolds themselves represent a sufficiently effective carrier for BMP-2. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00036838 |
| DOI: | 10.1134/S000368382470008X |
