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Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles.

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Title: Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles.
Authors: Krishna, Krishangi1 (AUTHOR) krishangi_krishna@brown.edu, Burrow, Joshua A.1 (AUTHOR) joshua_burrow@brown.edu, Diouf, Mbaye1 (AUTHOR) mbaye_diouf@brown.edu, Sun, Jieliyue1 (AUTHOR) jieliyue_sun@brown.edu, Harling, Mitchell1 (AUTHOR) mitchell_harling@brown.edu, Toussaint Jr., Kimani C.1,2 (AUTHOR) kimani_toussaint@brown.edu
Source: Optical Engineering. Feb2025, Vol. 64 Issue 2, p24107-24107. 1p.
Subjects: High power lasers, Microscopy, Femtosecond lasers, Biological specimens, Light intensity
Abstract: An optical tweezer (OT) platform based on light sheet microscopy with a continuous wave (CW) laser has been developed to trap multiple microscopic dielectric particles. However, the reduced gradient force resulting from the light sheet intensity distribution produces a trap stiffness an order of magnitude lower than its traditional circularly symmetric Gaussian counterpart. As a result, a high laser power on the order of 50 mW is required, which risks phototoxicity for biological applications. In addition, OT using 2D flat-top wavefronts has been shown to provide a more stable trap due to its steep intensity profile. The combination of flat-top beams and light-sheet techniques in OT significantly improves our ability to investigate and manipulate biological systems with exceptional precision and biological safety. Recently, we introduced femtosecond laser–assisted selective holding with ultra-low power (FLASH-UP), which enables the direct trapping of dielectric particles and bacteria using a 2D Gaussian wavefront at average powers sub-1 mW. To further elucidate the capabilities of FLASH-UP, we compare the OT applied to dielectric spheres using 2D flat-top, 1D light sheet, and 1D flat-top light sheet configurations generated by FLASH-UP to its CW counterpart utilizing average powers as low as 1 mW. Our findings demonstrate that FLASH-UP OT consistently generates higher trap stiffness than CW-OT. We propose leveraging flat-top light sheet OT to characterize the local and average mechanical properties of biological specimens. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:An optical tweezer (OT) platform based on light sheet microscopy with a continuous wave (CW) laser has been developed to trap multiple microscopic dielectric particles. However, the reduced gradient force resulting from the light sheet intensity distribution produces a trap stiffness an order of magnitude lower than its traditional circularly symmetric Gaussian counterpart. As a result, a high laser power on the order of 50 mW is required, which risks phototoxicity for biological applications. In addition, OT using 2D flat-top wavefronts has been shown to provide a more stable trap due to its steep intensity profile. The combination of flat-top beams and light-sheet techniques in OT significantly improves our ability to investigate and manipulate biological systems with exceptional precision and biological safety. Recently, we introduced femtosecond laser–assisted selective holding with ultra-low power (FLASH-UP), which enables the direct trapping of dielectric particles and bacteria using a 2D Gaussian wavefront at average powers sub-1 mW. To further elucidate the capabilities of FLASH-UP, we compare the OT applied to dielectric spheres using 2D flat-top, 1D light sheet, and 1D flat-top light sheet configurations generated by FLASH-UP to its CW counterpart utilizing average powers as low as 1 mW. Our findings demonstrate that FLASH-UP OT consistently generates higher trap stiffness than CW-OT. We propose leveraging flat-top light sheet OT to characterize the local and average mechanical properties of biological specimens. [ABSTRACT FROM AUTHOR]
ISSN:00913286
DOI:10.1117/1.OE.64.2.024107