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Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles.

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Title: Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles.
Authors: Krishna, Krishangi1 (AUTHOR) krishangi_krishna@brown.edu, Burrow, Joshua A.1 (AUTHOR) joshua_burrow@brown.edu, Diouf, Mbaye1 (AUTHOR) mbaye_diouf@brown.edu, Sun, Jieliyue1 (AUTHOR) jieliyue_sun@brown.edu, Harling, Mitchell1 (AUTHOR) mitchell_harling@brown.edu, Toussaint Jr., Kimani C.1,2 (AUTHOR) kimani_toussaint@brown.edu
Source: Optical Engineering. Feb2025, Vol. 64 Issue 2, p24107-24107. 1p.
Subjects: High power lasers, Microscopy, Femtosecond lasers, Biological specimens, Light intensity
Abstract: An optical tweezer (OT) platform based on light sheet microscopy with a continuous wave (CW) laser has been developed to trap multiple microscopic dielectric particles. However, the reduced gradient force resulting from the light sheet intensity distribution produces a trap stiffness an order of magnitude lower than its traditional circularly symmetric Gaussian counterpart. As a result, a high laser power on the order of 50 mW is required, which risks phototoxicity for biological applications. In addition, OT using 2D flat-top wavefronts has been shown to provide a more stable trap due to its steep intensity profile. The combination of flat-top beams and light-sheet techniques in OT significantly improves our ability to investigate and manipulate biological systems with exceptional precision and biological safety. Recently, we introduced femtosecond laser–assisted selective holding with ultra-low power (FLASH-UP), which enables the direct trapping of dielectric particles and bacteria using a 2D Gaussian wavefront at average powers sub-1 mW. To further elucidate the capabilities of FLASH-UP, we compare the OT applied to dielectric spheres using 2D flat-top, 1D light sheet, and 1D flat-top light sheet configurations generated by FLASH-UP to its CW counterpart utilizing average powers as low as 1 mW. Our findings demonstrate that FLASH-UP OT consistently generates higher trap stiffness than CW-OT. We propose leveraging flat-top light sheet OT to characterize the local and average mechanical properties of biological specimens. [ABSTRACT FROM AUTHOR]
Copyright of Optical Engineering is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 185072546
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles.
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  Data: <searchLink fieldCode="AR" term="%22Krishna%2C+Krishangi%22">Krishna, Krishangi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> krishangi_krishna@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Burrow%2C+Joshua+A%2E%22">Burrow, Joshua A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> joshua_burrow@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Diouf%2C+Mbaye%22">Diouf, Mbaye</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mbaye_diouf@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Sun%2C+Jieliyue%22">Sun, Jieliyue</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jieliyue_sun@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Harling%2C+Mitchell%22">Harling, Mitchell</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mitchell_harling@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Toussaint+Jr%2E%2C+Kimani+C%2E%22">Toussaint Jr., Kimani C.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> kimani_toussaint@brown.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Optical+Engineering%22">Optical Engineering</searchLink>. Feb2025, Vol. 64 Issue 2, p24107-24107. 1p.
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  Data: <searchLink fieldCode="DE" term="%22High+power+lasers%22">High power lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Microscopy%22">Microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Femtosecond+lasers%22">Femtosecond lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+specimens%22">Biological specimens</searchLink><br /><searchLink fieldCode="DE" term="%22Light+intensity%22">Light intensity</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: An optical tweezer (OT) platform based on light sheet microscopy with a continuous wave (CW) laser has been developed to trap multiple microscopic dielectric particles. However, the reduced gradient force resulting from the light sheet intensity distribution produces a trap stiffness an order of magnitude lower than its traditional circularly symmetric Gaussian counterpart. As a result, a high laser power on the order of 50 mW is required, which risks phototoxicity for biological applications. In addition, OT using 2D flat-top wavefronts has been shown to provide a more stable trap due to its steep intensity profile. The combination of flat-top beams and light-sheet techniques in OT significantly improves our ability to investigate and manipulate biological systems with exceptional precision and biological safety. Recently, we introduced femtosecond laser–assisted selective holding with ultra-low power (FLASH-UP), which enables the direct trapping of dielectric particles and bacteria using a 2D Gaussian wavefront at average powers sub-1 mW. To further elucidate the capabilities of FLASH-UP, we compare the OT applied to dielectric spheres using 2D flat-top, 1D light sheet, and 1D flat-top light sheet configurations generated by FLASH-UP to its CW counterpart utilizing average powers as low as 1 mW. Our findings demonstrate that FLASH-UP OT consistently generates higher trap stiffness than CW-OT. We propose leveraging flat-top light sheet OT to characterize the local and average mechanical properties of biological specimens. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Optical Engineering is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1117/1.OE.64.2.024107
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: 24107
    Subjects:
      – SubjectFull: High power lasers
        Type: general
      – SubjectFull: Microscopy
        Type: general
      – SubjectFull: Femtosecond lasers
        Type: general
      – SubjectFull: Biological specimens
        Type: general
      – SubjectFull: Light intensity
        Type: general
    Titles:
      – TitleFull: Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles.
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            NameFull: Krishna, Krishangi
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            NameFull: Burrow, Joshua A.
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            NameFull: Diouf, Mbaye
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            NameFull: Sun, Jieliyue
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              M: 02
              Text: Feb2025
              Type: published
              Y: 2025
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              Value: 64
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