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Target-induced DNAzyme recycle amplification strategy for colorimetric detection of cystatin C.

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Title: Target-induced DNAzyme recycle amplification strategy for colorimetric detection of cystatin C.
Authors: Lin, Pin-Yu1 (AUTHOR), Wang, Yi-Shan1,2 (AUTHOR), Ip, Im-Fong1 (AUTHOR), Chang, Chia-Chen1,2,3 (AUTHOR) chang@cgu.edu.tw
Source: Microchemical Journal. Jul2025, Vol. 214, pN.PAG-N.PAG. 1p.
Subjects: Gold nanoparticles, Cystatin C, Nucleic acid hybridization, Acute kidney failure, Deoxyribozymes, Creatinine
Abstract: [Display omitted] • A dual DNAzyme-amplified assay was developed for the detection of cystatin C. • Non-cross-linking gold nanoparticle aggregates were used to induce color signals. • The aptasensor demonstrated high selectivity for detecting cystatin C over other common kidney biomarkers. • The aptasensor effectively detected cystatin C in the serum sample. Cystatin C (Cys C) is an early biomarker of declining kidney function and is frequently detected during clinical diagnosis. However, existing assays are complex, lengthy, and expensive. To overcome these limitations, a dual DNAzyme-amplified assay for Cys C detection was developed using non-cross-linked aggregates of gold nanoparticles (AuNPs). Target-induced assembly of the two DNAzymes triggers a cascade of cleavage reactions, releasing complementary strands that hybridize with detection probes linked to the AuNPs, leading to enhanced plasmonic signals. The assay demonstrated a linear detection range of 2–32 ng/mL, with a detection limit of 1.1 ng/mL. Furthermore, the proposed method exhibited high specificity for Cys C detection, acceptable intra-assay precision, and robust stability. The assay was successfully applied to detect Cys C in serum samples, highlighting its potential for application in the diagnosis of acute kidney injury. The entire process, including aptamer biorecognition, DNA hybridization, and catalytic cleavage of DNAzymes, was performed in a homogeneous solution, thereby rendering the method highly straightforward and exhibiting high reproducibility. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:[Display omitted] • A dual DNAzyme-amplified assay was developed for the detection of cystatin C. • Non-cross-linking gold nanoparticle aggregates were used to induce color signals. • The aptasensor demonstrated high selectivity for detecting cystatin C over other common kidney biomarkers. • The aptasensor effectively detected cystatin C in the serum sample. Cystatin C (Cys C) is an early biomarker of declining kidney function and is frequently detected during clinical diagnosis. However, existing assays are complex, lengthy, and expensive. To overcome these limitations, a dual DNAzyme-amplified assay for Cys C detection was developed using non-cross-linked aggregates of gold nanoparticles (AuNPs). Target-induced assembly of the two DNAzymes triggers a cascade of cleavage reactions, releasing complementary strands that hybridize with detection probes linked to the AuNPs, leading to enhanced plasmonic signals. The assay demonstrated a linear detection range of 2–32 ng/mL, with a detection limit of 1.1 ng/mL. Furthermore, the proposed method exhibited high specificity for Cys C detection, acceptable intra-assay precision, and robust stability. The assay was successfully applied to detect Cys C in serum samples, highlighting its potential for application in the diagnosis of acute kidney injury. The entire process, including aptamer biorecognition, DNA hybridization, and catalytic cleavage of DNAzymes, was performed in a homogeneous solution, thereby rendering the method highly straightforward and exhibiting high reproducibility. [ABSTRACT FROM AUTHOR]
ISSN:0026265X
DOI:10.1016/j.microc.2025.114039