View in EDS

Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A.

Saved in:

Bibliographic Details
Title:	Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A.
Authors:	Notaro, Anna¹ (AUTHOR), Sturiale, Luisa² (AUTHOR), Palmigiano, Angelo² (AUTHOR), Garozzo, Domenico² (AUTHOR), Speciale, Immacolata¹ (AUTHOR) immacolata.speciale@unina.it
Source:	Carbohydrate Research. Sep2025, Vol. 555, pN.PAG-N.PAG. 1p.
Subjects:	Distribution (Probability theory), Lipopolysaccharide structure, Gram-negative bacteria, Polysaccharides, Species distribution
Abstract:	Delftia acidovorans is a Gram-negative bacterium traditionally considered as an environmental organism, although it has recently been identified as an opportunistic pathogen in immunocompromised patients. Furthermore, this bacterium was also recognized as part of the crypt-specific core microbiota in healthy mice. Despite its detection in various contexts, the chemical structure of its lipopolysaccharide (LPS) remains uncharacterized. Here, the structure of O-antigen and lipid A from Delftia acidovorans were characterized by combining chemical approaches with spectroscopic and spectrometric studies. In detail, the O-antigen is found to consist of a novel glycan sequence composed of 3)-α- l -rhamnose-(1→4)-α- d -glucose-(1→ disaccharide in the linear backbone, where glucose is in turn branched at the O-2 position with a β- d - N -acetyl-glucosamine, that is non-stoichiometrically acetylated at position O-6. Whereas lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution of acyl chains. [Display omitted] • Delftia acidovorans a bacterium isolated from crypt-specific core microbiota of healthy mice. • Structural determination of O-antigen and lipid A from Delftia acidovorans. • O-antigen consists of a novel glycan sequence. • Lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution. [ABSTRACT FROM AUTHOR]
	Copyright of Carbohydrate Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database:	Engineering Source

Description
Abstract:	Delftia acidovorans is a Gram-negative bacterium traditionally considered as an environmental organism, although it has recently been identified as an opportunistic pathogen in immunocompromised patients. Furthermore, this bacterium was also recognized as part of the crypt-specific core microbiota in healthy mice. Despite its detection in various contexts, the chemical structure of its lipopolysaccharide (LPS) remains uncharacterized. Here, the structure of O-antigen and lipid A from Delftia acidovorans were characterized by combining chemical approaches with spectroscopic and spectrometric studies. In detail, the O-antigen is found to consist of a novel glycan sequence composed of 3)-α- l -rhamnose-(1→4)-α- d -glucose-(1→ disaccharide in the linear backbone, where glucose is in turn branched at the O-2 position with a β- d - N -acetyl-glucosamine, that is non-stoichiometrically acetylated at position O-6. Whereas lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution of acyl chains. [Display omitted] • Delftia acidovorans a bacterium isolated from crypt-specific core microbiota of healthy mice. • Structural determination of O-antigen and lipid A from Delftia acidovorans. • O-antigen consists of a novel glycan sequence. • Lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution. [ABSTRACT FROM AUTHOR]
ISSN:	00086215
DOI:	10.1016/j.carres.2025.109586