Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A.
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| Title: | Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A. |
|---|---|
| Authors: | Notaro, Anna1 (AUTHOR), Sturiale, Luisa2 (AUTHOR), Palmigiano, Angelo2 (AUTHOR), Garozzo, Domenico2 (AUTHOR), Speciale, Immacolata1 (AUTHOR) immacolata.speciale@unina.it |
| Source: | Carbohydrate Research. Sep2025, Vol. 555, pN.PAG-N.PAG. 1p. |
| Subjects: | Distribution (Probability theory), Lipopolysaccharide structure, Gram-negative bacteria, Polysaccharides, Species distribution |
| Abstract: | Delftia acidovorans is a Gram-negative bacterium traditionally considered as an environmental organism, although it has recently been identified as an opportunistic pathogen in immunocompromised patients. Furthermore, this bacterium was also recognized as part of the crypt-specific core microbiota in healthy mice. Despite its detection in various contexts, the chemical structure of its lipopolysaccharide (LPS) remains uncharacterized. Here, the structure of O-antigen and lipid A from Delftia acidovorans were characterized by combining chemical approaches with spectroscopic and spectrometric studies. In detail, the O-antigen is found to consist of a novel glycan sequence composed of 3)-α- l -rhamnose-(1→4)-α- d -glucose-(1→ disaccharide in the linear backbone, where glucose is in turn branched at the O-2 position with a β- d - N -acetyl-glucosamine, that is non-stoichiometrically acetylated at position O-6. Whereas lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution of acyl chains. [Display omitted] • Delftia acidovorans a bacterium isolated from crypt-specific core microbiota of healthy mice. • Structural determination of O-antigen and lipid A from Delftia acidovorans. • O-antigen consists of a novel glycan sequence. • Lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution. [ABSTRACT FROM AUTHOR] |
| Copyright of Carbohydrate Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 186498653 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Notaro%2C+Anna%22">Notaro, Anna</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sturiale%2C+Luisa%22">Sturiale, Luisa</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Palmigiano%2C+Angelo%22">Palmigiano, Angelo</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Garozzo%2C+Domenico%22">Garozzo, Domenico</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Speciale%2C+Immacolata%22">Speciale, Immacolata</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> immacolata.speciale@unina.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Carbohydrate+Research%22">Carbohydrate Research</searchLink>. Sep2025, Vol. 555, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Distribution+%28Probability+theory%29%22">Distribution (Probability theory)</searchLink><br /><searchLink fieldCode="DE" term="%22Lipopolysaccharide+structure%22">Lipopolysaccharide structure</searchLink><br /><searchLink fieldCode="DE" term="%22Gram-negative+bacteria%22">Gram-negative bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Polysaccharides%22">Polysaccharides</searchLink><br /><searchLink fieldCode="DE" term="%22Species+distribution%22">Species distribution</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Delftia acidovorans is a Gram-negative bacterium traditionally considered as an environmental organism, although it has recently been identified as an opportunistic pathogen in immunocompromised patients. Furthermore, this bacterium was also recognized as part of the crypt-specific core microbiota in healthy mice. Despite its detection in various contexts, the chemical structure of its lipopolysaccharide (LPS) remains uncharacterized. Here, the structure of O-antigen and lipid A from Delftia acidovorans were characterized by combining chemical approaches with spectroscopic and spectrometric studies. In detail, the O-antigen is found to consist of a novel glycan sequence composed of 3)-α- l -rhamnose-(1→4)-α- d -glucose-(1→ disaccharide in the linear backbone, where glucose is in turn branched at the O-2 position with a β- d - N -acetyl-glucosamine, that is non-stoichiometrically acetylated at position O-6. Whereas lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution of acyl chains. [Display omitted] • Delftia acidovorans a bacterium isolated from crypt-specific core microbiota of healthy mice. • Structural determination of O-antigen and lipid A from Delftia acidovorans. • O-antigen consists of a novel glycan sequence. • Lipid A is predominantly represented by a hexa-acylated species with a symmetric distribution. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Carbohydrate Research is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.carres.2025.109586 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Distribution (Probability theory) Type: general – SubjectFull: Lipopolysaccharide structure Type: general – SubjectFull: Gram-negative bacteria Type: general – SubjectFull: Polysaccharides Type: general – SubjectFull: Species distribution Type: general Titles: – TitleFull: Unveiling the structure of Delftia acidovorans Lipopolysaccharide: insights into O-antigen and lipid A. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Notaro, Anna – PersonEntity: Name: NameFull: Sturiale, Luisa – PersonEntity: Name: NameFull: Palmigiano, Angelo – PersonEntity: Name: NameFull: Garozzo, Domenico – PersonEntity: Name: NameFull: Speciale, Immacolata IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 00086215 Numbering: – Type: volume Value: 555 Titles: – TitleFull: Carbohydrate Research Type: main |
| ResultId | 1 |
