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Rapid and sensitive on-site detection of peach (Prunus persica) species in fruit juices using a RAA-CRISPR/Cas12a approach.

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Title: Rapid and sensitive on-site detection of peach (Prunus persica) species in fruit juices using a RAA-CRISPR/Cas12a approach.
Authors: Wang, Lin1,2 (AUTHOR), Xing, Ran-Ran1 (AUTHOR) xingranrancaiq@163.com, Liang, Xiao-Ke1 (AUTHOR), Yu, Yao-Xian1 (AUTHOR), Yi, Qiu-Ju1 (AUTHOR), Lu, Si-Yuan1 (AUTHOR), Deng, Ting-Ting1 (AUTHOR), Zhang, Jiu-Kai1 (AUTHOR), Wang, Xu2 (AUTHOR), Chen, Ying1 (AUTHOR) chenyingcaiq@163.com
Source: Food Control. Dec2025, Vol. 178, pN.PAG-N.PAG. 1p.
Subjects: Quality control inspectors, Food adulteration, Food safety, CRISPRs, Fruit juices
Abstract: Food adulteration, particularly involving allergenic ingredients like peach (Prunus persica), poses risks to consumer health and food safety, yet current laboratory-based DNA detection methods require extensive processing time, specialized thermal cyclers, and trained personnel, limiting their field deployment. To address these constraints, we have developed a rapid (50 min), highly sensitive (10−2 ng/μL; 1 % 1 % (w/w) adulteration) and portable DNA assay by integrating recombinase-aided amplification (RAA) with CRISPR/Cas12a under a constant 37 °C isothermal step, with visual readout by naked eye. Incorporation of a self-heating patch replaces laboratory heating devices, enabling fully power-free on-site operation. In a comparison with real-time PCR, the assay demonstrated 100 % agreement, detecting unlabeled peach content in 25 % of commercial fruit juices, highlighting the assay's potential for routine market surveillance. This cost-effective, equipment-minimal approach empowers regulatory inspectors and quality control officers to perform rapid on-site authenticity checks, thereby strengthening food safety monitoring and consumer protection. • Rapid detection of peach DNA using RAA-CRISPR/Cas12a in 50 min. • Visual results without specialized equipment, with detection limits of 1 % adulteration. • Self-heating patch enables on-site detection without power or lab devices. • 100 % agreement with real-time PCR in commercial juices detecting. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Food adulteration, particularly involving allergenic ingredients like peach (Prunus persica), poses risks to consumer health and food safety, yet current laboratory-based DNA detection methods require extensive processing time, specialized thermal cyclers, and trained personnel, limiting their field deployment. To address these constraints, we have developed a rapid (50 min), highly sensitive (10−2 ng/μL; 1 % 1 % (w/w) adulteration) and portable DNA assay by integrating recombinase-aided amplification (RAA) with CRISPR/Cas12a under a constant 37 °C isothermal step, with visual readout by naked eye. Incorporation of a self-heating patch replaces laboratory heating devices, enabling fully power-free on-site operation. In a comparison with real-time PCR, the assay demonstrated 100 % agreement, detecting unlabeled peach content in 25 % of commercial fruit juices, highlighting the assay's potential for routine market surveillance. This cost-effective, equipment-minimal approach empowers regulatory inspectors and quality control officers to perform rapid on-site authenticity checks, thereby strengthening food safety monitoring and consumer protection. • Rapid detection of peach DNA using RAA-CRISPR/Cas12a in 50 min. • Visual results without specialized equipment, with detection limits of 1 % adulteration. • Self-heating patch enables on-site detection without power or lab devices. • 100 % agreement with real-time PCR in commercial juices detecting. [ABSTRACT FROM AUTHOR]
ISSN:09567135
DOI:10.1016/j.foodcont.2025.111502